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胞质

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The major changes include sparse and short and loose microvilli of endodermal epithelium cells, a reduction in apical microvesicles and subapical storage vacuoles, swilling of the mitochondria and a reduction or disappear in its cristea, a decrease in rough endoplasmic reticulum, abnormation in pattern and structure of nucleus, an increased vacuolation in cells, an extend in the interval of adjacent cells.

电镜观察发现,剂量在50g/mL以上时,YS上皮细胞微绒毛稀疏变短,胞质中贮液泡减少,线粒体、内质网等内膜系统发生多种细胞病理学改变,细胞间隙增宽,连接复合体破坏。

As a sequence, the cell evolved an adaptively coordinated response to limit further accumulation of unfolded proteins by expressing large amount of chaperons. In this pathway, Ite1, an ER transmembrane protein kinase/endoribonuclease, is essential for viability during ER stress.

当内质网腔内未折叠蛋白增多时,通过Irelp胞质面部分的丝氨酸/苏氨酸自磷酸化,诱导UPR特异性转录因子Haclp mRNA剪接成熟。

It is composed of lobules of pale-staining cells with abundant lipid in their cytoplasm.

它由胞质内有丰富脂质的明细胞小叶组成。

Transmission electron microscopy demonstrated that fibroblast was full of endoplasmic reticulums that cisternal-like expanded in the rhEGF nanoparticle group, and collagen proteins were synthesized in cavity.

透射电镜下可见rhEGF纳米微球组成纤维细胞胞质内有大量的内质网,扩张成池状,腔内充满了合成的胶原蛋白。

Results: Embryoid bodies grew well in Collagen Gel, the differentiated cells grew into the gel gradually; electronmicroscope showed that some induced cells were rich in Golgi's bodies and endoplasmic reticulums, a few secretory granules were also visible, which was similar to human adult thyrocyte.

结果 拟胚体在鼠尾胶原上生长状态良好,分化细胞贴壁后逐渐陷入鼠尾胶原;电镜下诱导细胞高尔基体、内质网丰富,部分诱导细胞胞质内见不典型分泌颗粒,与人成体甲状腺细胞超微结构类似。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后,先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时,此时卵母细胞已进入第一次减数分裂的前中期,并且在DIC倒置显微镜下可以看到浓缩的染色体,用Polscope可以发现明显的纺锤体,借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换,经过三次直流电脉冲作用后,分别有91.6%的胞质—MI核质体对融合,经过进一步的培养后,超过80%的重组卵母细胞排出第一极体,体外受精后分别有79%和77%的重组卵形成双原核,受精后的胚胎在KSOM胚胎培养液中体外培养4天后,超过60%的胚胎发育至囊胚。

Most sperm nuclei eventually developed into the male pronuclei, just like the normal sperms, except sperm nuclei in a few zygotes kept dense throughout the fertilization process. Dense chromosome body was seen at the nuclear area during the prophase of first mitosis, and in the middle of spindle at metaphase of first mitosis.

精子入卵后形成精核,大多能够逐渐解凝、液化并膨大,最终形成形态正常的雄性原核,但在第一次卵裂早期退化成浓缩的染色质小体,并在胞质分裂时随机地分配到其中一个子细胞里的分裂沟附近。

The permanent wilting point is reached when the plant has wilted to a point where it will not recover, even if more water is added, because the cells have plasmolyzed.

当植物的萎蔫达到一定程度就不可以再复原,这一极限被称为永久萎蔫点。超过此极限,补充再多水分也不能被吸收,因为已经胞质浓缩已经到达质壁分离的程度。

Results After the treatment with 15 μmol·L~(-1) of evodiamine for(24 h,)A375-S2 cells exhibited typical characteristics of apoptosis:loss of membrane microvilli,cytoplasmic hyp...

结果经15μmol.L-1吴茱萸碱处理24 h的A375-S2细胞表现出典型的凋亡特征,即细胞表面微绒毛消失、胞质空泡化、染色质浓集、边聚。

LH plaies a role to delay the occurrence of germinal vesicle, prolong the maturation duration of cytoplasm, enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro, and finally improve the potentiality of their in vitro development.

LH可能通过延迟生发泡破裂和促进胞质成熟的方式,使绵羊卵母细胞的核质成熟趋于同步,有助于绵羊卵母细胞的体外成熟以及受精后的进一步发育。

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