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胞浆素

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Results After oral administration of ST pinocytosis vesicles began to increase in endothelial cells of blood vessel in 1 hour,apocrine of ependymal cells began to increase and myelin sheath degeneration appeared in 2 hours,extensive myelin sheath degeneration and mitochondrion degeneration and incisure and vacuoles in nucleus of neuron were found in 4 and 8 hours.

结果 ST灌胃后1 h血管内皮细胞吞饮囊泡开始增多,2 h组室管膜细胞的顶浆分泌增多,部分神经髓鞘变性;4 h和8 h组神经纤维广泛变性,神经元线粒体变性、核切迹和空泡出现;16 h组神经元胞浆内线粒体变性更加明显,并出现脂褐素。

In our previous work, we found that RIG-G could interact with JAB1 and sequestrate the later in the cytoplasm, preventing JAB1 from facilitating the phosphorylated p27 nuclear export and degradation via the cytoplasmic ubiquitin/ proteasome pathway.

在以前的工作中,我们曾发现RIG-G可以与JAB1蛋白发生相互作用,并将后者阻滞在细胞浆中,无法正常携带磷酸化的p27蛋白出核,从而抑制了p27蛋白通过胞浆的泛素/蛋白酶体系统降解。

In this study, we investigated platelet functional changes in vitro when platelets were exposed to pharmacological concentrations of tissue-type plasminogen activator.

在本实验中,我们以溶血栓试剂组织胞浆素原活化因子(Tissue-type plasminogen activator;t-PA)体外处理血小板,并观察血小板功能的变化。

The pseudopodial movement could be inhibited by calcitonin (100 μg*L-1). Lacuna formation could be found in cocultured specimens. There were fibrils on the walls of lacunae. The MGCs demonstrated positive reaction with Gomori stain. Conclusion: The results demonstrated that MGCs had the same morphological features and the bone resorption ability as the osteoclasts.

结果:倒置相差显微镜下可见多核巨细胞胞核较多(20个以上),胞浆周边不规则,有伪足样突起;胞浆内可见较多大小不等的空泡;降钙素(100 μg*L-1)可抑制多核巨细胞的伪足样运动;多核巨细胞与灭活的骨片共同培养时可见骨吸收陷窝形成,扫描电镜下可见吸收陷窝底面有原纤维;Gomori染色时可见多核巨细胞的酸性磷酸酶呈阳性。

This study was to investigate the in situ localization of tissue type plasminogen activator in pulpal disease.

胞浆素原活化剂主要含有组织型(tissue-type plasminogen activator,简称t-PA)及尿激型(urokinase-type)的胞浆素原活化剂。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化;采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察;光镜观察输卵管粘膜上皮细胞的形态学变化。结果:1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论:成骨细胞呈梭形,多突起;细胞核呈卵圆形,偏于一侧;胞体大,胞浆丰富;碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒,某些部位染成黑色,定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞;细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

LC3-FITC had been used to monitor autophagy by immunofluorescence. It showed that there are a high level of LC3 puncta in HCQ+DOX or BAF +DOX treated cells, and in rapamycin treated cells, moderate level in HCQ, BAF, or DOX singly treated cells, and low level in 3-MA+DOX, and there was diffuse fluorescence in untreated or 3-MA treated cells.

内源性LC3免疫荧光染色显示,羟氯喹+阿霉素、BAF+阿霉素及雷帕霉素处理组MM细胞胞浆可见大量颗粒状荧光,羟氯喹、BAF、阿霉素单用组仅有中等量颗粒状荧光,而3-MA及3-MA+阿霉素组胞浆荧光信号基本呈弥散分布,类似于未处理对照组。5。

The plasminogen/plasmin proteolytic system participates in a wide variety of extracellular matrix degradation.

纤维蛋白溶解系统的胞浆素具有造成细胞间质裂解,产生组织破坏的特性,可能是牙髓致病路径之一。

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