胞浆分离

Results High homogenous hMSCs were obtained and after co-culture for 5 d with osteoblasts, they fused to form multinuclear cell clusters in micrograph and were postive in TRAP staining. Conclusion The co-culture system method is successful.

结果：体外分离、培养的hMSCs具有独特的细胞免疫学表型，即CD73和CD105阳性， CD34和CD45 阴性。hMSCs与成骨细胞共同培养5 d后，即可见单个核细胞融合成多核细胞，TRAP 染色可见多数细胞胞浆呈酒红色，为诱导成功的破骨细胞。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中，DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F，以重组质粒DNA和脂质体共转染COS-7细胞，用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

The HUVECs retained spindled shaped or cobblestone appearance and positively stained by anti-CD31. DAPI staining showed that 90％of scaffolds surface was reendothelialized and endothelial cells were distributed evently on the surface forming a confluent monolayer.

脱细胞支架细胞成分完全脱去，胞外基质保存完好；分离的细胞培养一周后，细胞呈梭形或多角形，核仁清晰，大小均匀，呈典型的铺路石样；免疫荧光检测可见细胞胞浆内有颗粒状红色阳性信号，说明Ⅷ因子相关抗原染色阳性；膜表面有蓝色阳性信号，说明CD31抗原染色阳性。

METHODS HP strains were obtained by culture from patients Vero cell intracytoplasmic vacuolization was induced by Broth Culture Filtrate ( BCF, 0.22um pore filter) of 43 of 62 HP strains obtained from patients.

62株临床分离的幽门螺杆菌，43株的肉汤培养滤液(0.22um微孔滤膜)作用于体外培养的Vero细胞，能使其产生胞浆空泡样变。

Pathologic examination included: tubulointerstitial lesion which was the main pathologic presentation, tubular epithelial cell becoming flat, TEC necrosis and wither, departure between the cytoplasm and basis membrane, drops of protein in cytoplasm, broken basic membrane of TEC, inflammatory cells infiltrating in tubulointerstitium, tubulointerstitium fibrosis, plasm cell infiltration.

MM肾损害主要以小管-间质病变为主，病理表现为肾小管上皮细胞扁平，存在不同程度变性、坏死或萎缩，胞浆与基膜分离，胞浆内可见蛋白滴，甚至小管基膜断裂，间质炎性细胞浸润、纤维化，部分有淀粉样物质沉积，较少见浆细胞浸润。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。