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胞浆

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Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

In control group, the expression of STAT3 protein in cultured hypothalamic cell was positive, but phospho-STAT3 protein expression was negative.

Leptin干预24 h后,培养下丘脑神经元的胞核和胞浆内均有磷酸化STAT3蛋白的表达,呈棕黄色。

Results After oral administration of ST pinocytosis vesicles began to increase in endothelial cells of blood vessel in 1 hour,apocrine of ependymal cells began to increase and myelin sheath degeneration appeared in 2 hours,extensive myelin sheath degeneration and mitochondrion degeneration and incisure and vacuoles in nucleus of neuron were found in 4 and 8 hours.

结果 ST灌胃后1 h血管内皮细胞吞饮囊泡开始增多,2 h组室管膜细胞的顶浆分泌增多,部分神经髓鞘变性;4 h和8 h组神经纤维广泛变性,神经元线粒体变性、核切迹和空泡出现;16 h组神经元胞浆内线粒体变性更加明显,并出现脂褐素。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法:48小时后见大量上皮细胞贴壁,细胞不规则呈圆形、椭圆形、多边形,细胞体肥大透亮,细胞核位于中央,胞膜清楚,约12-14天后细胞呈镶嵌状排列,融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

So, the nucleocytoplasmic ratio decreased. 10^(-5)MATRA significantly suppressed the proliferation of os732 with only 39.5% cells when compared with untreated cells. Cells in G1 phase increased significantly, and the number of S phase decreased. Expressions of cy-clinD1, cycimD2 and CDK4 mRNA declined, especially cyclinD2 and CDK However, expressions of cyclinD3 and P21WAF1 mRNA changed little.

结果 ATRA作用后,OS732细胞胞浆展开,细胞核变小,核浆比例变小;瘤细胞生长减慢,细胞计数为正常对照组的39.5%; G0/G1期细胞明显增多,S期细胞减少,细胞阻滞于G0/G1期;CyclinD1、CyclinD2、CDK4mRNA表达均下降,尤以CylinD2、CDK4下降最明显,CyclinD3、P21WAF1mRNA未见明显改变。

The staining pattern was cytoplasmic and/or membranous.

DOG1.1表达的阳性部位为胞浆或/和胞膜。

There has been positive fiber in the neurofibra of spiral ganglion and in the cochlea nerve of modiolus by ANP immunological reaction.

大多数螺旋神经节细胞为ANP免疫反应阳性细胞,棕色ANP免疫反应产物散布在胞浆内,少数神经节的胞核也被阳性染色。

NF-κ BP65 was mainly stained in cytoplasm or nucleolus.

NF-κ BP65主要分布在胞浆或胞核内呈棕黄色。

Results:(1) NPY protein was expressed in cell plasma.

结果:(1)NPY表达于胞浆或胞膜上。

Results: The four components of uPA system were expressed in the plasma and membrane of the four cell lines.

结果:4种成分在4株细胞的胞浆及胞膜均有表达。

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