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胞浆

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2 The result of electron microscope cytochemistry stain: The positive production of ACPase was present black lead phosphate deposit with high electron-dense under light microscope, located in cytoplasm or apophysis mainly. Lysosome was located in cytoplasm in the earlier of differentiation, then was present in apophysis with it grew.

4.2 电镜酶细胞化学染色结果:电镜下ACPase阳性产物为电子致密度高的黑色磷酸铅沉淀,主要分布于神经元的胞浆中或突起内,神经元分化的初期,溶酶体集中分布于胞质内,随突起的长出,可见在突起内出现。

The result showed that there was no expression in the normal control group and an apparent expression of ERK1/2 and Elk in the asthma group. A rather dense expressing of ERK1/2 was at bronchiole and mucous membrane, sub mucous membrane, smooth muscle, cytoplasm and nuclei of the out layer of the smooth muscle cell and an expression of positive fiber at submucous membrane.

结果发现正常肺内没有发现ERK1/2和Elk的表达,而哮喘时肺内有明显的ERK1/2和Elk表达,ERK1/2较密集表达在小支气管和细支气管的粘膜层、粘膜下层、平滑肌层和平滑肌外层细胞的胞浆和胞核中,也可见粘膜下层有阳性纤维表达。

And its expression in cytoplasm and nuclei was defined as ectopic expression.

而在胞浆和/或胞核表达为异位表达。

Results We found that the CLC-3 protein mainly expressed in the cytoplasm and on the cellular membrane.

结果 CLC-3蛋白主要表达在瘤细胞的胞浆和/或胞膜上。

ResultsThe expression of STAT 4、STAT 6 were both positive and located mainly in the decidua cells.

结果① 对照组和URSA组蜕膜组织中STAT 4、STAT 6均呈阳性表达,阳性染色主要位于蜕膜细胞的胞浆,胞核多数无明显着色。

In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形,细胞及胞膜完整,包质内可见丰富的粗面内质网、高尔基体、线粒体,细胞核完整,染色质分布均匀;模型对照组软骨细胞明显固缩且外形不规则,细胞周晕消失,胞浆内细胞器凝成高电子密度的片状物不易分辨,细胞核不规则,染色质浓聚,散裂于核中。

Results Of 40 cases of choroidal malignant melanoma, C-myc positive expressions were present diffusely in the cytoplasm or nucleolus of cancer cells in 20 cases.

结果 40例脉络膜恶性黑色素瘤标本中,C-myc在20例肿瘤的细胞胞浆或胞核中表达,阳性率为50%。

On the membrane of liver parenchymal cell and endothelial cell ×12 000;Fig 4 3 h after Cd-injection:ER extremely dilatant,mitochondria swollen,a lot of Ce-H2O2 precipitation on the membrane of PC,EC and Kupffer'scell ×15 000;Fig 5 6 h after Cd-injection:in irreversible state,hepato-cytoplasm clear,few organelle,a few Ce-H2O2 precipitation on the membrane of PC and eC ×5 500;Fig 6 24 h after Cd-injection:hepatocyte plasmotorrhexis,membrane of

ce呈点状沉积在肝实质细胞、内皮细胞膜上×12 000;图4 染镉后3小时:ER极度扩张,线粒体膨胀,内外膜模糊不清;见大量Ce沉积在PC、EC和Kupffer's细胞膜上×15 000;图5 染镉后6小时:处于不可逆改变状态的肝细胞胞浆清亮,少有细胞器;仍可见少量Ce沉积在PC和EC膜上×5 500;图6 染镉后24小时:可见破碎的肝细胞,胞内膜结构大多已不清晰;未见Ce的沉积×7 000

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

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