胞外酶

The results showed that the mutant contained high trehalose content and high cell survival rate kept even after 4 h 18 %vol alcohol treatment (no respiration deficiency existed), which indicated that high trehalose content could protect cells membrane and prevent DNA loss of mitochondria and exosmosis of intracellular materials.

结果表明，中性海藻糖酶缺失突变株细胞海藻糖含量较高，用18 %vol的酒精处理4 h后仍可保持高的细胞存活率，并且无呼吸缺陷型的出现，说明高海藻糖含量可以保护细胞膜，防止线粒体DNA丢失和胞内物质的外渗，与酵母的酒精耐性之间存在一定的关系。

The clathrin triskelions assenble into a basket-like convex framework that causes the membrane to invaginate clathrin-coated vesicles migrate into the cell where the clathrin coats are lost before delivering their contents to the lysosomes.

网格蛋白三脚复合体装配成篮状凸起懂得框架，使得膜在该处内陷，最终出芽而形成小泡。而在胞吞作用中，这些网格蛋白包被的小泡转移到细胞中，此处网格蛋白外被在小泡的内含物转移到溶酶体之前即行消失。

Methods:Using cell culture and immunohistochemical techniques,the peripheral blood specimens from 33 RA patients were designed to test the human T cell lymphotropic virus type Ⅰ related antigens,reverse transcriptase and spontaneous syncytium formation.

采用细胞培养、免疫组织化学技术等对33例RA患者的外周血样本进行人类嗜T淋巴细胞病毒Ⅰ型相关抗原、逆转录酶活性测定及观察自发性合胞体形成。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果：1、通过不同感染途径的实验证实，滴鼻感染的小鼠主要表现为间质性肺炎，而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现，随着感染Q热立克次体剂量的加大，动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现，在腹腔注射后第2d的脾和肝脏即可发现病原体，主要脏器的病理变化从第2d到第12d逐渐加重，第7d动物的病变最典型，至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统，在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内，呈多形性，多见圆形和杆状，大小不一。

Results:(1)The primary cells in cultured were observed from the edge of the cultured tissues after 10-15 days. The cultured cells were round , oval, fusiform and multiangular.

结果(1)组织块培养10-15天后可见细胞从其边缘向外生长，倒置显微镜下呈梭形、圆形、椭圆形，并有多个突起，电镜下可见表面有较多的微绒毛，细胞间缝隙连接，胞质内见较多次级溶酶体。

When HIV-1 in culture medium was exposed to a range of concentrations of urokinase, a dose-dependent inhibition of infectivity was observed in a variety of lymphoma and leukemia cell lines including MT4, CEM, H9, and peripheral blood mononuclear cells using assays for reverse transcriptase, P24 antigen and syncytia formation.

通过检测逆转录酶活力，P24抗原的表达和合胞体形成情况发现尿激酶可以抑制人体免疫缺损病毒对多种淋巴瘤和白血病细胞系，如MT4、CEM、H9和外周血单核细胞的侵染能力，并且这种抑制与尿激酶浓度呈剂量依赖关系。

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞，加入莫能霉素，用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间，比较其胞内表达白细胞介素－2(IL-2)/白细胞介素－4(IL-4)的细胞百分率，确定最适诱生条件；用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验，确认McAb、bio-SAM IgG和HRP-SA的最适浓度，根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。