胞外酶

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Having been induced by cicada exuviae inducer ［(ρ/g L-1): cicada exuviae 2, chitin 2, K2HPO4 0.3,NaCl 0.3,MgSO4.7H2O 0.3,add 1 L water］ for 12 h, germ tube produced greater amount of total extracellular protease as well as Pr1 than other combination of inocula and inducers.

其中芽管状物在蝉蜕诱导液中的总胞外蛋白酶和Pr1活性均最高，诱导12 h酶活性达高峰。

This strain can produce the strong fibrinolytic enzyme and a large of mucilage.

该菌株的重要特点是产生强力纤溶酶和大量胞外粘质，后者经鉴定为γ-多聚谷氨酸。

Culture conditions were optimized to produce the extracellular alginate-lyase by the single factor and orthogonal tests.

通过单因子和正交试验对LJ1 菌株产胞外褐藻胶裂解酶的培养条件进行了优化。

The in vivo competitive binding test was study by adding the segment protein which CREG most binding to into the supernatant with wt/mCREG receptively. The change of CREG biologic effects on VSMC were analyseses to identified the direct binding of CREG to IGF2R domains.

将与wt/mCREG蛋白高亲合的M6P/IGF2R胞外重组小肽片段分别添加到细胞培养上清中，确定wt/mCREG蛋白对VSMC生物学行为的调控作用(流式细胞分析检测细胞增殖、刮伤实验和明胶酶谱分析细胞迁移能力)是否通过其与M6P/IGF2R小肽的直接亲合作用介导。

So we propose that before the precursor is cleaved into DG and mediator, it is released from plasma membrane first, through activating some kind of enzyme by insulin or PIPLC; meanwhile, only insulin can activate the resynthesis mechanism of the precursor, in the contrast, PIPLC was inactive at this course.

推测：胰岛素或PIPLC作用于完整细胞或膜时，激活了某种酶，使前体先从膜上释放至胞外，再被水解为介体和DG，同时胰岛素还能激活再合成前体的机制，而PIPLC却不能。

In this work, laccase was purified from the white rot fungus Cerrena unicolor LS0547 to evaluate its application in dye decolorization.

本文从白腐菌一色齿毛菌LS0547中纯化出胞外漆酶并用于染料脱色实验。

The glucoamylase I gene was inserted into the pAC1 plasmid,and a expression plasmid was constructed,then it was used to transform the yeast strain, S.cerevisiae AS2.1364,without auxtrophic marker,The amylolytic tests showed that when this transformant was incubated on plate of YPDS medium containing 1% glucose and 1% starch at 30℃ for 48 hrs zones of starch degradation could be visualized by stained with iodine vapour.

这说明酵母转化子不仅表达了该酶，且有效地分泌到胞外，从而水解培养基中的淀粉，进一步测定酵母转化子48hrs发酵液的葡萄糖淀粉酶活力为8.4u/ml，培养基中的淀粉已完全水解，其生物产量较高。

The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bocillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis.

结果：分离得到421株细菌，包括活性菌株54株，其中活性菌株32-11-2-2是一株弱嗜盐菌，具有陆地芽孢杆菌的形态特点，能产生胞外淀粉酶、过氧化氢酶，液化明胶、石蕊牛奶反应阳性，16S rDNA序列分析该菌株与枯草芽孢杆菌同源性达到98%以上。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。