胞基质

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Thus, Clara cells resemble alveolar type II and bronchiolar ciliated epithelial cells by showing integrin mediated pro-migratory changes to extracellular matrix components that are present in tissues after injury.

Clara细胞与肺泡II型上皮细胞和支气管纤毛上皮细胞类似，其组织损伤后向胞外基质的促迁移改变依赖于整合素的调节。

Effects of Croton tiglium total alkaloids on cancer cell cytoplasm membrane fluidity and structure of the cytoplasmic matrix .

巴豆总生物碱对癌细胞质膜流动性及胞浆基质结构的影响。

ObjectiveTo explore the methods of prevention and cure of sun-induced aging dermis, and clarify the mechanism of Ginsenoside Rb1 on sun-induced aging dermis, with the study of expression of matrix metallo preteinases in sun-induced aging dermis fibroblasts' cytoplast, which are cultured in vitro, under the effect of Ginsenoside Rb1 and arotinoid ethyl ester.

目的通过人参皂苷Rb1与芳维甲酸乙酯对皮肤真皮体外培养光老化成纤维细胞胞质内基质金属蛋白酶表达的影响，阐明人参皂苷Rb1对光老化的防治机理，探索光老化的防治手段。

Objective To explore the invading mechanism of amebae in lamina porpria and observe the interaction between the cysteine proteinase of Entamoeba histolytica and laminin.

目的 探索溶组织内阿米巴通过基底膜进入固有膜的机制，了解其半胱氨酸蛋白酶(cysteine pro-teinase，CP)与胞外基质的相互作用。

Some analyzations and comparisons were carried out which are the researching results of adhesion properties between different cancer cells and the extracellular matrix with the aid of micropipette aspiration technology.

对近年来我院应用微管吸吮技术进行的癌细胞与胞外基质的粘附行为研究结果进行了分析和比较。

Prussian blue staining showed numerous blue stained fine particles in the cytoplasm of 99% BMSCs.

普鲁士蓝染色结果显示99%以上骨髓基质细胞的胞质内出现细小的蓝色铁颗粒。

The experiments suggest a relationship between (1) the FN cytoplasm location and the invasion of trophoblastic tumor and the FN cytoplasm location and the trophoblastic migration during normal secondary gestations.

纤维粘连蛋白（fibronectin，FN）作为间质细胞的胞外基质成分研究较多，在胎盘的分布状况也较常见［1，2］，但对正常和异常滋养上皮的准确定位和功能的关系的全面系统报道研究未见报道。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。