胞基质

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果：诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

EGF was mainly located in the cytoplasm and extracellular matrix of intestinal villus cells, endothelial cells and tunica serosa epithelial cells, while EGFR chiefly distributed in the cellular membrane of these cells.

其中 EGF主要存在于小肠黏膜上皮细胞、黏膜下层的血管内皮细胞内和浆膜上皮细胞的胞浆和胞外基质中，EGFR则分布于这些细胞的细胞膜上。

RESULTS: After co-culture, morphology of bone marrow stromal stem cells was changed, showing that flat cells began to retract and became spindle-shaped and some cells displayed a linear arrangement like Schwann cells.

结果：共培养后的骨髓基质干细胞形态发生改变，原来宽大扁平的胞体开始收缩，细胞胞体呈梭形，部分细胞类似施万细胞呈线性排列。

Metalloproteinases, a family of zinc-dependent proteolytic enzymes, play an important role in degradation of extracullular matrix.

基质金属蛋白酶是一群依赖金属锌的酵素，其在一些胞外基质的分解过程中，也扮演了相当重要的角色。

RESULTS:①Matrix metalloproteinase-2 and matrix metalloproteinase-9 were found in vascular endothelial kytoplasm of pseudarthrosis.

结果：①基质金属蛋白酶2和基质金属蛋白酶9主要在血管内皮细胞胞浆中表达。

A part of microbial cells would autolyzed to release the intercellular alkanedegrading enzymes when they were cultured on alkane. Thus alkane could be degraded and synthesized alkane derivative with surface activity extracellularly. This surface-active agent would improve alkane emulsifying, and those unautolyzed cells could utilize the emulsified alkane sequentially.

微生物细胞在烃类基质中培养时，为了适应其疏水性环境以更好地摄取烷烃，会通过一部分细胞的自溶解体而使得胞内烃类代谢酶等释放，这样烃类在胞外被代谢生成具有表面活性的烷烃衍生物，该表面活性产物将促进烃类的乳化，使得剩余的未自溶解体的细胞可以借助生物表面活性剂而摄取降解烃类。

In-depth studies were carried out on the extracellular·OH scavenging effect of white rot fungus with various media conditions in the dynamic degrading process of lignocellulose, and on the correlation between·OH scavenging and lignocellulose degradation. The·OH scavenging efficiency of A3 reached 45%~50% significantly during biodegrading process.

对不同白腐菌、丝状真菌及细菌的木质纤维素基质液体培养体系，进行胞外·OH清除能力比较研究，结果表明：体系·OH清除能力在白腐菌类群中较为普遍，相对于细菌、丝状真菌等常见微生物群落则是较独特的，该能力与菌株木质纤维素降解机制及能力相关，受底物基质营养构成状况影响。

Objective: In study of atherosclerosis, extracellular matrix of plaque is closelyrelated to the evolution of AS ,the balance of MMPs and TIMPs is very important to maintain the dynamic equilibrium of ECM, and the creation of appropriate animal models of AS is the important prerequisite for studying pathogenesis of AS and therapeutic programs.

目的：在动脉粥样硬化的研究中，斑块的胞外基质成分的变化与疾病的进展有重要联系，而基质金属蛋白酶和金属蛋白酶组织抑制物的平衡对维持ECM的动态平衡有着十分重要的作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。