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胞内酶

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Results:(1)The primary cells in cultured were observed from the edge of the cultured tissues after 10-15 days. The cultured cells were round , oval, fusiform and multiangular.

结果(1)组织块培养10-15天后可见细胞从其边缘向外生长,倒置显微镜下呈梭形、圆形、椭圆形,并有多个突起,电镜下可见表面有较多的微绒毛,细胞间缝隙连接,胞质内见较多次级溶酶体。

The cell vacuolar degeneration, membrane structural necrosis, mitochondrin edema, mitochondrin vacuolar degeneration and lysosome myelinization were found in the endothelial cells.

1胞核内也可见纳米细菌,细胞空泡变,膜性结构类似坏死,部分线粒体水肿,空泡变,溶酶体呈现髓鞘图像。

Results: The Fos-like immunoreactive neurons distributed mainly in the spinal trigeminal subnucleus caudalisipsilaterally and medullary visceral zone (MVZ,including nucleus tractus solitarius,ventrolateral medulla and the reticular formation between the first two),ln the sections processed for double staining of Fos and TH immunohisto chemistry, numerous Fos-LI neurons showed also TH- like immunoreactivity in their cytoplasm(Fos-TH cells)in MVZ.

结果:Fos样免疫反应神经元主要分布在同侧三叉神经脊束核尾侧亚核、双侧延髓内脏带(孤束核、腹外侧区及二者之间的网状结构)。在抗Fos 和抗酪氨酸羟化酶的双重免疫染色切片上,见到延髓内脏带内许多Fos阳性神经元的胞浆同时呈TH样免疫反应(Fos-TH双重阳性细胞)。

The basolateral M cell surface is invaginated to form an intracellular central pocket into and out of which lymphocytes and macrophages.

M细胞是一种特化的上皮细胞,多分布于Peyer's结圆顶区的周边,FAE的顶端未见M细胞;其结构有别于相邻的肠上皮细胞,表面没有长而整齐的微绒毛,取而代之的是粗而短的微皱褶,胞质内终末网不发达,溶酶体较少,基底膜向顶部穹隆状突起,形成一&口袋&样结构,其中含有多个免疫活性细胞。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

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