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胞内酶

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The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果:在同一标本上,不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况,二者的分布区域大致相同,神经元分化初期,较集中分布在核周附近,神经元分化成熟则分布于胞质及突起内。

Results Under optical lens positive products of ACP reaction were brown particles scattered in cyton and process;under electron lens its positive products were black precipitates with high electron density scattered in lysosome and Golgi bodies in different shapes,and often companied by linear lysosome and some cell organs.

结果光镜下可见 ACP 反应阳性产物为棕色颗粒,散在分布于神经元的胞体及突起内;电镜可见其阳性产物为电子密度高的黑色沉淀,分布于不同形状的溶酶体及高尔基体内,并常见线状溶酶体与一些细胞器相伴行。

At early stage after brain injury, there were a quantity of fibrous callus and cartilaginous callus formation in brain injury and fracture group and many neuropeptides immunoreactive nerve fibers in callus were found. Strong immunoreactivites of CGRP, SP, VIP, NPY, TOH occurred to osteogenitor cells and chondroblast, which proliferated in thickened endothecium.

脑损伤合并骨折组早期形成大量纤维骨痂和软骨骨痂,骨痂中神经肽免疫阳性神经纤维较多,明显增厚的骨膜内层骨祖细胞、幼稚的软骨细胞胞质内降钙素基因相关肽、P物质、血管活性肠肽、酪氨酸羟化酶、神经肽Y强阳性表达。

During the secondary wall formation of fiber, H+-ATPase, Ca2+-ATPase and APase also sustainablely remained in plasma membrane, plasma membrane invagination, plasmodesmata, transfer vesicles and agglutinated nucleus.

在次生壁形成的整个过程中,ATP酶、Ca2+-ATPase和酸性磷酸酶在运输小泡、细胞膜、质膜内陷、胞间连丝和凝聚的染色质中将持续存在。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuo le membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中,质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物,线粒体和小泡上只有少量酶活性反应产物。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuole membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中,质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物,线粒体和小泡上只有少量酶活性反应产物。

Then histochemical, electron microscopic, enzyme histo cytochemical and confocal laser scanning microscopic methods were employed to study the distribution of vinculin, cathepsin Dand nematolysosomes ...

结果在正常对照组神经元,组织蛋白酶D与纽蛋白分布于胞质及突起内;在CD及PMA处理神经元,纽蛋白及组织蛋白酶D的分布呈向心性移动,但集聚的部位不同;电镜酶细胞化学方法显示CD组及PMA组神经元内线状溶酶体均增多。

Results①The Rab9 and NPC1 colocalized in cytoplasm of microglia.②Rab9 expressed in lysosome,Golgi apparatus and late endosome.

结果Rab9和NPC1共定位于小胶质细胞胞浆内,Rab9表达于溶酶体、高尔基体和晚期内体。

In order to understand whether and how Pos5p and Zwf1p affect the form and content of coenzymes, intracellular content of NAD+, NADH, NADP+ and NADPH in different stains were analysized by HPLC.

为了了解它们是否以及如何影响细胞内NAD类辅酶的形式及含量,我们利用HPLC法,对比分析了野生株和突变株胞浆内NAD+、NADH、NADP+和NADPH的含量。

The acidification of cytosl might inhibit furgher endocytosis.Alkalization of lysosomes was a basic factor for exocytosis of lysosomal contents.

胞浆pH的降低可能有抑制内吞的作用,溶酶体pH上升是触发溶酶体内容物外排的基本因素。

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