胞内酶

2 The result of electron microscope cytochemistry stain: The positive production of ACPase was present black lead phosphate deposit with high electron-dense under light microscope, located in cytoplasm or apophysis mainly. Lysosome was located in cytoplasm in the earlier of differentiation, then was present in apophysis with it grew.

4.2 电镜酶细胞化学染色结果：电镜下ACPase阳性产物为电子致密度高的黑色磷酸铅沉淀，主要分布于神经元的胞浆中或突起内，神经元分化的初期，溶酶体集中分布于胞质内，随突起的长出，可见在突起内出现。

The ultrastructure resultshowed that in model group blood intima was adema,broadening, evendiscontinuous and incomplete under blood vessel bast. In the blood themonocyte and the lymphocyte infiltrated to intension board. Manymacrophages could befound and there were massive all levels of dissolve theenzyme body and swallow slightly soak in its cytoblastema,The endosarc netand the ribose body was increased and outside the amnion the pseudopodappeared. Endothelial cell morphogenesis changed. It is obvious thatmitochondria was dropsy, cytoblastema was non-uniformity.

超微结构结果显示模型组血管内皮下内膜水肿、增宽，甚至间断不完整；血中单核细胞及淋巴细胞向内弹力板浸润，可见较多巨噬细胞，其胞浆内含大量各级溶酶体及吞噬小泡，内质网及核糖体增多，胞膜外出现伪足；内皮细胞形态发生改变，可见线粒体水肿，胞浆不均匀；中药治疗后，上述病理损害明显减轻。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。

The induced hmscs-tert were examined by oil red-o staining, alkaline phosphates staining and von kossa staining. results the hmsc-tert was negative for cd34, cd45, cd44, cd106 and cd166. after the induction, accumulated cytoplasmatic red lipid droplets were clearly visible in the adipose cells by oil red o staining, and alkaline phosphates-positive pitchy grains formed in the intracytoplasm and calcium nodes were found by von kossa staining in the osteoblasts.

结果 hmsc-tert细胞表面抗原检测cd34、cd45、cd44、cd106、cd166均为阴性；脂肪细胞定向诱导后油红o染色见胞浆内有红色脂肪颗粒，成骨细胞定向诱导后见胞浆内有碱性磷酸酶染色阳性黑褐色颗粒，von kossa染色见有钙结节形成。

The formation of silique dehsicence susceptibility was investigated in terms of water content, synthetic rate of ethylene, enzyme activity of exo polygalacturonase and end cellulase , cell alteration in dehiscence zone in silique and silique dehiscence susceptibility during silique development.

从油菜角果发育过程中角果含水量、乙烯合成速率、外切 PG 酶和内切纤维素酶活性，以及角果裂区切片观察等方面研究了角果开裂敏感性形成的原因⒚花后50d 的角果果柄端壳状果瓣和线状果瓣间的裂区薄壁细胞膨大，在高倍光学显微镜下可见胞间空隙开始形成⒚此前6d 内乙烯合成速率迅速上升并出现第二次合成高峰，与之同步发生外切 PG酶和内切纤维素酶活性急剧上升，而乙烯合成第二高峰出现于角果含水量低于80％以下⒚由此看来，角果失水引起乙烯合成第二高峰出现，从而导致水解酶活性上升，最终导致果瓣裂区薄壁细胞膨大，分离和胞间空隙形成及角果开裂敏感性产生

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的：从红花五味子果实中寻找活性代谢产物，研究其抗HIV—1活性和作用机制方法：通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验，检测分离化合物的抗HIV—1活性并探讨其作用机制一结果：红花五味子甲素能够抑制病毒诱导的合胞体形成，保护病毒感染细胞，抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^＋细胞间的融合，逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制，也不抑制蛋白酶的活性、结论：首次从天然产物中分离得到红花五味子甲素，它对HIV—1病毒复制早期具有抑制作用

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论：成骨细胞呈梭形，多突起；细胞核呈卵圆形，偏于一侧；胞体大，胞浆丰富；碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒，某些部位染成黑色，定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞；细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。