胞内酶

During the process of long-time adaptive evolution, wetland plants adopted a series of special strategies to acclimate to salt stress. The main strategies are: 1 life history adjustment, e. g., to adjust seed germination time, implement seed dormancy and viviparity, and change reproductive manner to escape from direct salt stress, 2 morphological adjustment, e. g., to adjust biomass allocation pattern, age stem, defoliate, and carnify vegetative organs to isolate the redundant Na(superscript +) to the inactive-metabolism shoots or exclude the Na(superscript +)from tissues; 3 anatomic adjustment, e. g., to sink stoma, develop aerenchyma, and thicken cuticle and phellogen to maintain normal photosynthesis and respiration; 4 physiological and biochemical adjustment, e. g., to exclude and excrete salt, compartmentalize ions, adjust osmosis, do selective absorption, regulate hormones, and induce antioxidative enzymes to maintain the osmotic equilibrium and eliminate the active oxygen in cell; and 5 molecular level adjustment, e. g., to start up many salt-induced genes to regulate the metabolic responses to salt stress.

在长期的适应进化过程中，湿地植物形成了多种适应盐胁迫的策略，主要有：1生活史方面，植物可通过种子萌发时间的调整、种子体眠、胎生、繁殖方式的改变等逃避盐度的直接伤害；2形态学方面，植物可通过生物量分配模式的调整、茎的老化、落叶及营养器官的肉质化等将多余的Na隔离到代谢不活跃的茎中或将其排出体外；3解剖学方面，植物可通过气孔下陷、发达的通气组织、增加细胞木栓层、角质层及栅栏组织的厚度等以维持植物正常的光合作用和呼吸作用；4生理生化方面，植物可通过离子区隔化、拒盐、泌盐、选择性吸收、渗透调节、激素调节及抗氧化物酶的诱导等来维持细胞内正常的渗透压，清除胞内活性氧分子；5分子水平方面，植物可通过多种与盐胁迫相关的基因来调控细胞内的多种代谢反应。

ABAS could be decolored by extracellular composite enzymes secreted by Zoogloea HP3. The degradation place of o-pathalic acid was in-cell. Compared with the degradation of o-pathalic acid, the decoloring of ABAS controlled the degradation of ABAS.

动胶菌HP3分泌的胞外组成酶可使溴胺酸脱色，溴胺酸降解中间产物邻苯二甲酸的降解场所在胞内；与邻苯二甲酸的降解相比，溴胺酸的脱色是反应的控制步骤。

In accordance with the present invention, optically pure-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give-hydroxycarboxylic acid.

根据本发明，光学纯的-羟基羧酸可通过培养用重组质粒转化的E.coli制备，所述重组质粒表达真养产碱杆菌的胞内PHA解聚酶和广泛产碱菌的PHA生物合成酶从而生成-羟基羧酸。

The physical and chemical properties of the purified MPH were studied. In the crude extracts, the specific activities of MPH for methyl parathion and fenitrothion were higher than those of OPH from F.sp, while the catalytical activity for parathion was lower than that of OPH.

我们根据Pseudomonas sp WBC-3的生长特性及产酶情况，以阳离子交换层析纯化胞内粗蛋白中，使酶的比活提高了49.1倍，得到了均一的目的蛋白MPH，SDS-PAGE验证为单一的条带，。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated by high performance liquid chromatography with differential refraction detection basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths.

通过高效液相色谱/示差折光检测系统分析可获得细菌D-97利用糊精或淀粉水解物合成海藻糖的基本生物学信息，包括微生物培养碳源对细菌D-97胞内海藻糖合成酶系的影响以及该酶系利用不同种类或不同分子链长度的麦芽寡糖合成海藻糖的能力及作用过程。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.

结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

Result］All of enzyme tests were normal.The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

Result］all of enzyme tests were normal.the author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察。［结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

A part of microbial cells would autolyzed to release the intercellular alkanedegrading enzymes when they were cultured on alkane. Thus alkane could be degraded and synthesized alkane derivative with surface activity extracellularly. This surface-active agent would improve alkane emulsifying, and those unautolyzed cells could utilize the emulsified alkane sequentially.

微生物细胞在烃类基质中培养时，为了适应其疏水性环境以更好地摄取烷烃，会通过一部分细胞的自溶解体而使得胞内烃类代谢酶等释放，这样烃类在胞外被代谢生成具有表面活性的烷烃衍生物，该表面活性产物将促进烃类的乳化，使得剩余的未自溶解体的细胞可以借助生物表面活性剂而摄取降解烃类。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。