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Morphorgenesis and differentiation of these organs involves sequential and complex interaction between ectoderm, mesoderm and endoderm.

这些器官的成型与分化,皆是由胚胎外、中、内三个胚层组织一连串的讯息传递以及彼此之间的交互作用所完成的。

Totipotent cells have the capacity to specialize into extraembryonic membra nes and tissues, the embryo, and all postembryonic tissues and organs.

全能细胞具有分化为特定的胚胎外膜和组织、胚胎以及所有胚胎后组织和器官的能力。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明:(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料;(2)在所试验到的MS、MS1/2(将MS的所有成分均减半)、WPM、DKW、B〓、SH等六种基本培养基中,MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基;(3)水曲柳的体细胞胚胎发生以直接发生为主,体细胞胚主要来自于从合子胚分离的完整子叶;(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响,诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2,5,10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0,2mg/L+TDZ 0.05mg/L;(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力,少数愈伤组织可以分化出体细胞胚;(6)愈伤组织的诱导也受培养基和激素配比的显著影响,最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2,5mg/L+TDZ0.05mg/L;(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力,此时种子尚未成熟,胚乳已呈固态,种胚的各个部分已分化完全,种胚体积占胚腔的大约2/3;(8)在各自综合的最适条件下,完整子叶的体细胞胚诱导率可达33.68%,下胚轴的愈伤组织诱导率可达90.7%。

Endoscopic Describing the type of development of a plant embryo in which the inner cell formed by the first division of the zygote develops into the embryo, while the outer cell develops into the suspensor.

内向极性:该名词用来描述植物胚胎发育的一种形式,指受精卵第一次分裂形成的内细胞最后发育为胚胎,外细胞发育为胚柄。

The results of study show:1. White pulp and red pulp in parenchyma of spleen of embryo can be obviously discerned after 18 days. Periarterial lymphoid sheath and ellipsoid periarterial lymphoid sheath also can be obviously discerned in spleen of 4 days chicken. T, B lymphocytes in appendix basement of embryo emerge after 20 days. It is the initial shape of cecal tonsil. Crypt structure of conjunction of esophago and stomachus glandularis form obviously at 4 days. It is the initial shape of esophago tonsil. The germinal center firstly emerges in these three organs at 14 days. With the increase of day age, the characteristic structure peripheral immune organs gradually develop mature. Spleen achieve mature at 21 days and cecal tonsil at 35 days.2. IgM~+ and IgA~+ cells in spleen of embryo emerge at 15 days. IgG~+ cell, CD3~+ and CD8~+T lymphocytes of embryo emerge at 20 days. CD3~+, CD8~+ and IgM~+ cells in cecal tonsil of embryo emerge at 20 days. However CD4~+, IgG~+ and IgA~+ cells all emerge in 1 day age chicken out of crust. CD3~+, CD4~+, CD8~+, IgM~+ and IgG~+ cells in esophago tonsil of embryo all emerge at 20 days. However IgA~+ cells emerge in 1 day age chicken out of crust.3. The amount of T, B lymphocytes in peripheral immune organs increase follow with the increase of day age, and hold an upgrade tendency. The amount of T, B lymphocytes in spleen achieved stabilization at 21 days, and in tonsil of esophago and appendix at 35 days.

研究结果表明:1、在组织结构方面,脾脏实质内的白髓与红髓在胚胎18日龄后明显可辨,4日龄雏鸡脾脏中形成明显可辨的动脉周围淋巴鞘和椭球周围淋巴鞘;盲肠基部T、B淋巴细胞在胚胎20日龄时开始出现,即初步形成盲肠扁桃体;食管与腺胃结合处在4日龄时形成明显的隐窝结构,即食管扁桃体初步形成;14日龄时,三种器官中首次出现生发中心;随着日龄的增长,外周免疫器官特征结构不断发育成熟,脾脏在21日龄时达到成熟水平,盲肠扁桃体和食管扁桃体在35日龄时达到成熟水平。2、在T、B淋巴细胞出现时间方面,脾脏中IgM~+和IgA~+细胞在胚胎15日龄时开始出现,IgG~+细胞、CD3~+和CD8~+T淋巴细胞在胚胎18日龄时出现,CD4~+细胞在胚胎20日龄时出现;盲肠扁桃体中CD3~+、CD8~+和IgM~+细胞在胚胎20日龄时开始出现,而CD4~+、IgG~+和IgA~+细胞均在雏鸡出壳后1日龄时出现;食管扁桃体中CD3~+、CD4~+、CD8~+、IgM~+和IgG~+细胞均在胚胎20日龄时开始出现,而IgA~+细胞则在雏鸡出壳后1日龄时出现。3、在T、B淋巴细胞数量变化方面,外周免疫器官中T、B淋巴细胞的数量随日龄增长,整体均呈上升趋势。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果: NKA-I细胞首先见于胚胎14 d的十二指肠粘膜,早于空肠(胚胎16 d)和回肠(胚胎17 d),其阳性反应强度及细胞量随发育而增加,30 d时与成年鼠相似;结肠粘膜内于胚胎17 d见细胞,但此后数量较少直至成年;胃粘膜于胚胎15 d见个别细胞外,以后无明显变化;食道壁始终未见阳性细胞。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果:NKA-I细胞首先见于胚胎14 d的十二指肠粘膜,早于空肠(胚胎16 d)和回肠(胚胎17 d),其阳性反应强度及细胞数量随发育而增加,30 d时与成年鼠相似;结肠粘膜内于胚胎17 d见多个细胞,但此后数量较少直至成年;胃粘膜于胚胎15 d见个别细胞外,以后无明显变化;食道壁始终未见阳性细胞。

Nes and tissues, the embryo, and all postembryonic tissues and organs.

全能细胞具有分化为特定的胚胎外膜和组织、胚胎以及所有胚胎

Objective : To measure the differences of total T cell percentages of peripheral blood, peripheral immune organs and central immune organs of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time, and to analyze the effects of these differences on interspecific embryo implantation.

目的:研究种间胚胎植入期母体外周血、外周免疫器官、中枢免疫器官中总T细胞的百分比变化,并探讨这种变化对种间胚胎植入的影响。

The results showed that the HRP-labelled pinellin was localized on the epitheliurri of endometrium and glandula as revealed after development with 3, 3'-diaminobenzidine–H_(2)O_(2), and the effect could be inhibited by unlabelled pinellin.

结果显示小鼠子宫内膜和腺管上皮以及胚胎外胚盘锥体部分呈现明显的酶标显色颗粒,它们的产生能被未经酶标半夏蛋白所抑制。

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