胚胎发育

This study was carried out to investigate whether the GV oocytes from carcasses could be utilized. Carcasses were preserved at 10℃, 15℃, 20℃ and 25℃ for 8 hours, 14 hours, 24 hours, or 48 hours after mice were killed and GV occytes were collected. After maturating in vitro, these oocytes were fertilized in vitro with the general procedure in advance.

将ICR系雌性小鼠处死并在10℃、15℃、20℃和25℃下依次保存8、14、24和48h后，采集其体内的卵巢GV期卵，采用常规方法进行体外成熟和体外受精，获得的2细胞期胚经体外培养或胚胎移植观察其发育能力。

Indirect immunofluorescence was used to observe the distribution of fibronectin in human oocyte and 2-cell to morulae embryos.

纤维粘连蛋白均匀分布在体外未受精人卵母细胞和受精后人胚胎早期发育的不同阶段的卵裂球表面，各时期的免疫荧光强度未见明显差别。透明带未见纤维粘连蛋白表达。

Toxicity tests showed dextran and trehalose solutions had no effects on in vitro development of morulae after exposed for 5-20 mm at 250C.

抗冻保护剂溶液对桑椹胚的毒性试验证明，25℃下，胚胎在葡聚糖和海藻糖溶液中平衡5-20分钟，对其体外发育无不良影响。

The rate of morulae/blastocysts was (59.5±3.2)%(P.05). In conclusion, the sequence culture system (SOF and granulosa cell+NCSU-23 FCS and POEC) can improve the development of procine parthenogenetic embryos.

结果表明，SOF培养基和颗粒细胞＋添加胎牛血清的NCSU-23和输卵管上皮细胞的序贯培养系统能较好的促进胚胎的发育。

When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.22%, the highest rate in all groups. After the morulae cultured from thawed embryos from the best straw group and OPS group were transferred, all groups have obtained offspring.

在25℃温度条件下细管法和OPS二步法玻璃化冷冻后的小鼠2-细胞胚胎，经培养发育至囊胚后，用H33342染色，在荧光显微镜下观察并进行细胞计数。

Methods C 57BL/6J strain female mice mated with KM male mice, after seeing plug 3.5 da ys the compacted morulae and blastocysts were collected and bisected microsurgic ally by using special knife, obtained half embryos were cultured and observed.

C57BL/6J雌性小鼠与KM雄性小鼠交配，见栓3.5d采集小鼠晚期桑椹胚和早期囊胚，应用显微手术刀直接分割法进行胚胎二等份分割，所得半胚体外培养，观察其发育情况。

At the earlier period of pregnancy, before the formation of blood vessels, embryogenesis precesses in the hypoxia environment.

低氧作为一种生理性的刺激因素，影响着胚胎的发生、发育及正常功能的维持。

Transplantation of motoneurons derived from MASH1-transfected mouse ES cells reconstitutes neural networks and improves motor function in hemiplegic mice.

ES细胞向神经细胞分化的研究有利于人们了解胚胎神经细胞发育的机制、发现ES细胞向神经细胞分化的关键调节基因；建立体外定向诱导神经细胞分化体系将为神经疾病治疗药物的筛选以及基因工程药物的开发等提供有用的材料；体外大量有功能的特定神经元的获得使神经再生医学正一步步从理论走向实践。

It is known that one of the important mechanisms for epigenetic reprogramming is DNA methylation.

目前的研究认为这些异常是由于体细胞核在去核卵母细胞中重编程错误造成的，导致克隆牛胚胎的发育潜能降低。

Methods and Results-: A total of 45 human embryonic, fetal, and neonatal sectioned hearts (4 to 36 weeks of development) were studied immunohistochemically.

方法和结果：共有45个人类胚胎、胎儿和新生儿的心脏切片（发育4至36周）用免疫组织化染色进行研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。