胚胎发育

Methods: With the techniques of Immunohistochemistry, Immunocytochemistry, in situ hybridization and Rat vibrissa follicles culture in vitro.,we studied the expression and role of FGF-13 in skin follicles during three different biological processes.

首先对胚胎生长发育过程FGF-13在皮肤及毛囊中的表达进行了检测。

The ruthless winnowing of inappropriate offspring can, it seems, also take place well after a fetus has started developing.

这个残忍的筛选后代的过程看来可以是在一个胚胎已经开始发育后发生的。

Effect of ethanol on the development of mouse visceral yolk sac.

乙醇对小鼠胚胎脏层卵黄囊发育影响的体外研究。

Three types of cells were transferred into perivitelline space under zona pellucida of rabbit oocytes respectively.

用体外培养14代和24代的小鼠胚胎干细胞构建的异种重构卵的卵裂率分别是88.5％和82.1％(P＞0.05)，囊胚发育率分别是16.7％和15.4％(P＞0.05)。

The stages of development for a baby are zygote, embryo, and fetus.

婴儿的发育阶段有受精卵、胚胎、然后是胎儿。

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

The study, led by Benjamin Alman from the Hospital For Sick Children, Toronto, inestigated the role of the β-catenin signaling pathway, which actiates T cell factor -dependent gene transcription, and which is known to hae a key regulatory role in embryonic skeletal deelopment.

这项由来自多伦多儿童医院（Hospital For Sick Children？）的Benjamin Alman领导的研究调查了β连环蛋白信号通路的作用，这条通路能激活T细胞因子依赖的基因转录，并且被发现在胚胎骨骼发育中起关键作用。

The main research achievements on oogenesis and embryogenesis in mammals are described in this review,including the resumption of meiotic division;activation of MⅡ oocyte;development of parthenogenetic,gynogenetic,androgenetic and reconstituted embryos;and the genetic and reproductive potentials of chromosomes within the first and the second polar body.

本研究将论述哺乳动物卵子发生和胚胎发生的最新研究进展，包括：减数分列的启动， MⅡ期卵母细胞的激活，孤雌胚、雌核胚、雄核胚和重组胚的发育以及第一与第二极体染色体的遗传和生殖潜力。

Then, under the control of systemic factors, mesenchymal cells aggregate and differentiate to form catilage blastemal elements that prefigure skeletal limb components.

脊椎动物胚胎期骨与关节系统的发生是一种复杂生命现象，起始于中胚层间充质细胞的定向聚集，形成肢芽，然后在一系列作用因子的调控下，肢芽内细胞进一步分化，形成具有骨骼雏形的软骨原基，后者经软骨内骨化发育成骨。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。