胚胎发育

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上，用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻，胚胎解冻后，囊胚发育率分别为：90％、90.1％、89.2％、91.4％；移植后着床率分别：50.0％、47.1％、43.4％、46.5％，差异不显著；并且与对照组相比差异也不显著。

To investigate the expression of VEGF, KDR and CD34 in human yolk sac at different stages, we applied immunohistochemical staining and light microscope.

为研究胚胎不同时期VEGF、KDR和CD34在人卵黄囊中的表达情况，了解胚胎造血的发育过程和机理。

To understand the factors effecting on mammalian early embryo development, we carried out the study through 3 points of view, oocytes, zona and embryo culture in vitro.

本研究分别从卵母细胞、透明带以及胚胎体外培养三个角度来探讨影响哺乳动物早期胚胎体外发育的因素。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

Therefore, a experimentent with three exposure types (early cleavage exposure, 0hpf; gastrula exposure, 8hpf and 0hpf exposure but stop after 8 hours, 8h stop) were designed to investigate the toxicity and bioaccumulation of bisphenol A in zebrafish embryo in different stages.

为研究胚胎在不同发育阶段对外源化合物的毒性敏感差异，以0hpf、8hpf和8h终止3种暴露方式进行染毒，探讨斑马鱼胚胎在不同暴露时段对双酚A毒性和生物蓄积特征。

Therefore, a experimentent with three exposure types (early cleavage exposure, 0hpf; gastrula exposure, 8hpf and 0hpf exposure but stop after 8 hours, 8h stop) were designed to investigate the toxicity and bioaccumulation of bisphenol A in zebrafish embryo in different stages.

为研究胚胎在不同发育阶段对外源化合物的毒性敏感差异，以0hpf、8hpf和8h终止3种暴露方式进行染毒，探讨斑马鱼胚胎在不同暴露时段对双酚A。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

Furthermore, we investigated the dynamic characteristics of lamin B by immunofluorescence during the nuclear envelope reconstruction of PM-embryos.

本研究表明：在去除抑制剂3h后未能形成核膜的胚胎进一步卵裂，而形成核膜的PM胚胎培养24h未见进一步发育。

As far as it is known from the history of embryo logy, little was known about the staging and classification of human embryo s until the twentieth century.

就胚胎学的历史而言，在20世纪之前，人们对人类胚胎的发育阶段和分类都知知甚少。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。