胚胎发育

Based on the above results, we can conclude that Mist1 functions in an evolutionary conserved way as a key transcriptional regulator specific for exocrine pancreas development in zebrafish. In addition, it is involved in the embryonic CNS development, and even earlier initial period of development.

根据以上结果可以得出结论，Mist1作为转录因子，以一种进化保守的方式特异性调控斑马鱼外分泌胰腺的发育；另外，它还在斑马鱼胚胎早期发育过程中参与了中枢神经系统的发育，以及更早的发育起始过程。

We generated EG4-GFP cell lines expressing enhancing green fluorescence protein which still maintained typical characteristics of pluripotent stem cells. We microinjected green cells into perivitelline of 8-cell stage embryos as well as aggregated them to 8-cell stage embryos with zona pellucida removed, to investigate developmental fate of EG4-GFP cells during formation of chimeric blastocysts.

我们建立了稳定表达绿色荧光蛋白的EG细胞系（EG4-GFP），发现该细胞仍能保持野生型的一般生长特性；通过显微注射和体外聚集得到细胞与8-细胞受体胚胎共同发育形成的嵌合胚，发现转基因细胞参与了胚胎早期发育过程中的生长与分化，大部分细胞集中于内细胞团，也有细胞分化为滋胚层细胞。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析，结果显示：(1)小鼠卵母细胞的乙醇激活结果表明，随着乙醇浓度的升高和作用时间的延长，卵母细胞激活率和发育率都显著提高，但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加，7%乙醇作用卵母细胞7min 为最佳激活条件。

Tolerance threshold of 2-cell mouse embryos to ethanol was around 1.5% when these embryos were cultured in Whitten's medium with 0, 0.1%,0.5%,1.0%, 1.5%, 2.0%, 3.0%, 5.0% and 10.0% ethanol, respectively.

用含不同浓度乙醇的Whitten氏培养液对小鼠2细胞、4细胞、8细胞和桑椹期胚胎分别进行体外培养，研究了乙醇对小鼠不同发育时期胚胎体外发育的影响。

During somatic embryogenesis and zygotic embryos development,the start-up and morphogenesis of embryos are regulated by a variety of factors.In particular,somatic embryogenesis is a temporal and special regulated developmental process.

在体细胞胚胎发生和合子胚发育的过程中，无论是胚胎发生的启动还是胚状体的形态建成都受到多种因子的调控，体细胞胚发生是一个由多种基因参与的复杂时空调节的发育途径。

Other ground had reported that the level of mouse bex1 mRNA differenced with the development stage of testis, but nothing had been known about its functions in brain. We cloned the mouse Bex1 gene and characterization of its expression pattern in the mouse central nervous system.

我们首先克隆得到小鼠的Bex1基因，检测小鼠不同发育时期胚胎中Bex1mRNA的表达变化情况，发现它在成年鼠脑中的表达量大大低于小鼠胚胎，说明它确实与小鼠脑的发育相关。

Owing to placenta and milk transshipping effect, the content of Aroclor 1254 in fetal and infant vivo was very low, so there were not significantly pathological changes in sexual gland, the effect of Aroclor

2首次以排卵数、受精率、受精卵的第一次卵裂率、桑堪胚发育率、囊胚~孵化胚发育率、以及发育延迟胚和退化变性胚的比率，对个体发育的不同时期接触不同剂量 AroClor 1254对小鼠卵子发生、受精和附植前胚胎体内发育毒性进行系统定量。

But embryo got through 2-cell block after H2O2 treatment occurred compensatory growth in later development, there were no differences with the embryo cell number and the cleavage index compared with normal developmental embryo.

但是经H_2O_2处理之后渡过2-细胞期阻滞的胚胎能在以后的发育中发生补偿性生长，其胚细胞数和分裂指数都和体外正常发育的胚胎没有差异。

Coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

子宫内膜上皮细胞能够促进小鼠胚胎的体外发育，对早期胚胎的体外发育环境具有优化作用。

At the same concentration of aluminum,the total Brown's morphogenetic score reflecting the every index of embryo growth and development and organ morphogenetic differentiation,the incidence rates of terata and the number of dead embryos were significant lower in rats exposed to stored solution compared with those exposed to newly prepared solution,especially the morbidity of embryos.

在同一浓度下，给予存放溶液的大鼠其反映胚胎生长发育的各项指标和反映器官形态分化的Brown总评分，以及畸形胚胎发生率和胚胎死亡均明显低于新溶液，其中胚胎死亡率下降幅度更大。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。