胚胎发育

AIM: To detect the expressions of insulinlike growth factorⅠ in villus and IGFII in decidua and the levels in mother serum, and to explore their relationship with the embryogenesis of early pregnancy.

目的：探讨胰岛素样生长因子I和II在正常胚胎发育及早期胚胎发育终止绒毛及蜕膜组织中的表达及其在母体血中水平的改变与胚胎发育的关系。

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1～8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

结果：①0.1ng/ml、1ng/ml和10ng/ml浓度的LIF对小鼠早期胚胎的发育和胚泡的形成有促进作用，0.01ng/ml和100ng/ml浓度的LIF对胚胎的发育无明显影响；②LIF对小鼠早期胚胎发育的影响主要发生于桑椹胚和胚泡期，对1～8细胞期胚胎无明显影响。

Besides, the lengths of siliques at different developing stages were measured, and relation between silique length and time course were investigated, the results showed that the siliques grew slower as time goes on.

此外还测量了胚胎不同发育时期的角果长度，获得了胚胎发育时期与角果长度之间的对应关系，发现随着胚胎的发育，角果的生长速率呈现出逐渐递减的趋势。

The content variation to Chrysopa septempunctata"s vitellin was detected through ELISA. It indicated that vitellin was utilized gradually during embryonic development, and a fallowing trend in form of straightline was found in the whole embryonic development. Vitellin couldn"t be detected in just hatched larvae.

通过ELISA来测定大草蛉的胚胎发育和胚后发育中卵黄蛋白的含量变化，随着胚胎的发育，卵黄蛋白逐渐被利用，在整个胚胎发育过程中呈直线下降趋势，在刚孵化的幼虫中已检测不到卵黄蛋白的存在。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

Relationship between longans embryo development and changes of polyaminesThe thesis also studied the relationship between longan embryo development and contents of polyamines . Experiments showed that the contents of polyamines in normal ovules were higher than those in abortive ones during the whole embryogenetic process.

龙眼胚胎发育与多胺的变化研究了龙眼胚胎发育与多胺含量的关系，结果表明：正常发育的胚珠中多胺含量在胚胎发育的各个时期均高于败育胚珠。

Opportunistically expressed (usually at the basal levels of transcription), but still permit the continued expression of genes that are regulated by strong promoters/enhancers (such as housing-keeping gene), the expresison of these genes might play a key role in the embryonic development; 5 The sum of these two opposing processes-activation and repression-would dictate the final pattern of gene expression that manifests itself as a dramatic reprogramming of gene expression during the maternal-to-zygotic transition and support further embryonic development.

这些基因的表达对于胚胎的后继发育可能是至关重要的；5）通过胚胎基因表达的激活与抑制：这两个作用相反的机制依不同时间顺序协同作用于胚胎基因，从而在指导胚胎发育的控制权由母源性表达产物移交到胚胎特异的基因表达产物的过程中&再程序化&胚胎基因的表达，形成特定的表达模式，以支持胚胎的后继发育。

BACKGROUND: At present, studies show that a kind of stem cell community which in many kinds of organizations can differentiate into tissue cells of different embryonic layers; but those are different from embryonic stem cells, embryonic stem cell will lose the part differentiation potential gradually during the development of pregnancy, and will present some special phenotypes or the molecular markers, as CD105 and so on, will call it postembryonic pluripotent stem cells.

背景：目前研究认为在胚胎发育后的多种组织中存在一类干细胞群体，可分化为不同胚层的组织细胞；但又不同于胚胎干细胞，随着妊娠期间胚胎的发育胚胎干细胞会逐渐失去部分分化潜能，会出现一些特殊表型或分子标记，如CD105，称其为胚胎后亚全能干细胞。

In this study, we used mRNA Differential Display to analysis the change of gene expression pattern of mouse embryo in the above two stages and the effect of releasing the transcriptionally repressive state on the embryonic development.

为了研究胚胎发育早期基因表达模式的变化及其生理意义，我们使用mRNA差异显示的方法分析了小鼠胚胎基因组激活初期（1-2细胞胚胎）表达的mRNA及随后在二细胞胚胎中转录呈抑制状态的基因表达产物，并探讨了解除二细胞胚胎中转录抑制状态对胚胎后继发育的影响。

The Institute for Stem Cell Research is primarily dedicated to research and education in stem cell including developmental immunology, embryonic patterning, developmental epigenetics, cardiovascular stem cell, embryo stem cell neurogenesis, developmental haematopoiesis, embryo stem cell biology, genome engineering, bioinformatics, early embryo development, etc.

爱丁堡大学干细胞研究所主要致力于干细胞的研究和教学，其学科领域包括发育免疫学，胚胎模式，发育遗传学，心脏血管干细胞，胚胎干细胞神经发生，发育造血作用，胚胎干细胞生物学，基因组工程，生物信息学，早期胚胎发育等。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失