胚胎

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Mutants withdefects in epiboly, axis, somite, head, and cardiac and blood systems were identifiedby observing morphological changes in F3 embryos. Additionally, in situhybridization was adopted to identify mutant lines with altered expression patterns ofdpr2, frb35 and insulin.

通过对 F3 代胚胎的表型观察，鉴定了在外包、体轴、体节、头部、心血管系统等表现异常的突变体；通过原位杂交的方法，筛选了 dpr2，frb35 和胰岛素基因表达异常的突变体。

A genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage Transcriptswith at least two-fold changes in expression level between wildtype and the other samples were identifyied In squint mRNA-injected embryos, 265 transcripts show anincreased expression level and 111 have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level.

为鉴定受Nodal信号调控的基因，特别是那些转录因子基因，通过将来自squint过量表达、缺失Nodal信号的MZoep突变体或野生型30%外包期胚胎的RNA与Affymetrix斑马鱼寡核苷酸芯片杂交。

We found that TN-C was abundant in the early heart development of rats, it was suggested that TN-C reference to the early heart morphogenesis; TN-X was poorly expressed in epicardium in hearts of ED11.5, implied it may have something to do with the formation of the coronary artery.

TN-C在大鼠胚胎心脏发育早期表达丰富，对心脏的早期发育有一定关系；而TN-X在ED11.5于形成血管的心外膜细胞周围表达，提示TN-X可能与心脏早期冠脉等血管的形成有关。

In this study,the expression and role of PAI-3 in the epithelization of human skin,including normal adult human skin,cultured differentiated keratinocytes and the development of human embryo,were observed,so as to elucidate the possible role of PAI-3 in the epithelization of skin and provide the experimental base for further studying the mechanism in epithelization of skin and all kinds of skin diseases.

本实验旨在研究正常成人皮肤组织及培养分化KC 中和胚胎生长发育过程表皮KC 中的PAI-3 的表达及作用，希望有助于阐明PAI-3 在皮肤上皮化中的功能，为进一步研究PAI-3 在皮肤上皮化中的机制及各种皮肤疾病提供实验基础。

Objective:To study the proliferation and apoptosis of epithelia in the esophagus and intestines of fetal mice.

目的：研究小鼠胚胎食管和肠上皮细胞的增殖与凋亡。

For household users to establish simple antifreeze dilution disposal ways of applying gender-controlled embryo freezingly-preserved technique by one-step vitrification dilution method,A,B,and C structure approaches of liquid layers in canaliculi were applied to investigate their survival capacity after embryos freshly-split by one-step dilution;and minimum capacity refrigerant method in specific canaliculus and above structure approach of liquid layer B were also applied to compare their survival capacity of frozen and melted embryos freshly split,and frozen and melted IVF embryos splited by one-step dilution.

为了创立在用户庭院就能应用的玻璃化保存性别控制胚胎的冷冻剂稀释处理方法，采用细管内3种不同液体层构成法，对新鲜分割胚一步法稀释后的存活能力进行了研究；其次用特定细管最小容量冷却法与上述3种不同液体层构成中效果较好的B法对玻璃化冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚进行一步法稀释后的存活能力予以对比。

In order to study the mechanism by which estrogen effects on bone, the effect of estrone on expression of c-myc protein in the epiphyseal plate of fetal mouse long bone was detected by immunohistochemistry procedure in vitro.

为初步探讨雌激素对骨的作用机制，以体外培养的小鼠胚胎长骨作为模型，通过免疫组织化学方法检测雌酚酮对骺板静止区、增殖区和肥大区c-myc蛋白表达的影响。

The natural form of LIF is a 38 to 67 kDa glycosylatedprotein widely expressed in both adult and embryonic eutherian mammals .

LIF的天然形式是38-67kDa的糖基化蛋白，在哺乳动物的成年个体和胚胎中广泛表达。

In eutherian mammals, XCI is thought to be triggered by the expression of the non-coding Xist RNA from the future inactive X chromosome3, 4, 5; Xist RNA in turn is proposed to recruit protein complexes that bring about heterochromatinization of the Xi6, 7. Here we test whether imprinted XCI, which results in preferential inactivation of the paternal X chromosome, occurs in mouse embryos inheriting an Xp lacking Xist.

在最新一项研究中，美国北卡罗琳娜大学研究人员用小鼠胚胎（研究人员用基因工程方法使这些小鼠在其未来失活的X-染色体上含有一个有缺陷的Xist基因）所做的新的研究工作显示，实际情况更为复杂。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！