胚胎

The method of embryonic body formation was used for endoderm/mesoderm commitment before the following pax4 gene transfection.

首先使胚胎干细胞形成embryonic body，四天后将EB打成单颗细胞。

The scientists noted that specialized cells generated from the AFS cells included all three classes of cells found in the deeloping embryo - termed ectoderm, mesoderm, and endoderm.

科学家还发现从羊水衍生的干细胞中生长出来的特定的细胞包含了发育的胚胎的三种细胞类型——外胚层、中胚层和内胚层。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

Objective: To explore the expression of Slug in mouse endometrium during early pregnancy.

目的：初步探讨锌指转录因子Slug在早孕小鼠胚胎植入过程中的作用。

Objective To explore the relationship between vascular endothelial growth factor and development of human fetal bone.

目的 探讨血管内皮生长因子与人胚胎骨发生及发育的关系。

Results The development of colon ENS and colon wall has distinct stages.

结果 人胚胎结肠肠神经系统发育有明显的阶段性。

Porcine parvovirus is a major cause of reproductive failure in swine, manifested as embryonic resorption, fetal mummification, abortion and stillbirths .The virus is ubiquitous among swine throughout the world and is enzootic in most herds that have been tested.

猪细小病毒(porcine parvovirus，PPV)为引起母猪繁殖障碍的主要病原之一，猪细小病毒感染的主要特征是孕猪在怀孕前容易受到感染，可引起胚胎或胎儿的感染和死亡，导致母猪发生流产、死胎、胎儿木乃伊化及新生仔猪的死亡。

According to NIH team leader Ronald McKay, that means epiblast cells offer scientists a iew of an ES cell just as it's poised to produce mature cell types, including neurons, muscle and bone.

根据美国国家卫生所的研究小组负责人Ronald McKay的说法：这就意味着外胚层细胞给予科学家一种胚胎干细胞全新的观点，那就是他是一种制造成熟细胞类型，其中包括神经，肌肉和骨的一种平衡。

In the embryo, Nanog specifically demarcates the nascent epiblast, coincident with the domain of X chromosome reprogramming.

如果没有它，胚胎干细胞将不会发育，而诱导产生多功能干细胞的过程也会失败。

Recently, there is already known Pregeneolone (P5) which could affect epiboly movement of zebrafish embryos is the first product of the steroidogenesis pathway, but effects of further downstream products on epiboly movement are unknown.

目前研究已知固醇荷尔蒙生合成机制的第一个产物pregnenolone (P5)对斑马鱼胚胎epiboly 移动有影响，但其它下游的固醇荷尔蒙对epiboly移动的影响仍然未知。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！