胚胎

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内，在血管和淋巴管内皮细胞则无表达；VEGFR-3亦表现为广泛的表达，于血管和淋巴管内皮细胞可见阳性表达，并随着胚胎的发育，其在淋巴管内皮细胞的阳性表达率明显增加。

The morphological and spontaneous contractile properties of isolated cell appeared to be typical embryonic cardiomyocytes when assessed by phase-contrast microscope.

心肌肥大会使胚胎时期出现过的基因重新表达[5]，所以在胚胎心脏上曾经存在的电流可能会在病理情况下重新表现，从而导致某些心脏病的发生，事实上，在自发性高血压大鼠和人体衰竭心脏的心室肌细胞上都记录到了if [1， 2]。

Embryos were explanted and cultured 4 hours after CP administration. On d 10.5, culture was terminated and embryos were examined for yolk sac diameter, head length and crown-rump length and teratogenic effects of CP.

本文应用小鼠全胚胎培养技术，通过妊娠d 8分别ip 5，10，15和20 mg.kg-1环磷酰胺，研究了该药对器官原基形成期胚胎的致畸作用，并对其致畸机理作了初步探索。

The present study was conducted to investigate the effect of different culture systems, co-culture cells and their sequence culture on the in vitro development of porcine parthenogenetic embryos derived from in-vitro matured oocytes.

经体外成熟、孤雌激活和培养获得猪胚胎，研究了不同培养体系、共培养体细胞和序贯培养对猪孤雌激活胚胎发育的影响。

If the foetus is infected at less than 35 days of age, before there has been an opportunity for bone development, death results, followed by complete absorption and ultimately a small litter is born.

如果胚胎在35天之前感染猪细小病毒会发生胚胎死亡，由于此时胎儿的骨骼尚未发育完全，死胎随后将被完全吸收。结果会导致产仔数减少。

The explanation for this must lie in the biochemical maturity of eight-celled embryos compared with their junior, four-cell siblings.

对于这一现象只能解释为，在生化意义上发育至八个细胞的胚胎比只有4个细胞的初级胚胎要成熟。

In early development stage, muscarinic agonist carbachol significantly decreased the magnitude of the pacemaker current If but had no effect in late development stage.

我们发现，在胚胎发育的早期阶段，M胆碱能受体激动剂(muscarinic agonist carbachol，CCh)明显抑制If，但在胚胎发育的晚期阶段，CCh对If的抑制作用消失。

Methods: To use ECM Gel as a tissue culture substrate, 3-dimention co-culture of the choroids plexus or the Neuro-2A cells with the ventral part of the embryonic spinal motor column (Embryonic 12 days, E12) were made in the substrate.

采用ECM Gel作为组织培养的基质底物，将脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养，观察脉络丛组织或者Neuro-2A细胞株对胚胎脊髓运动神经元轴突生长的影响。

Objective To observe the effects of coculture of mouse embryo with endometrial epithelial cells on early embryonic development in vitro.

目的：采用子宫内膜上皮细胞与小鼠胚胎共培养，观察其对早期胚胎体外发育的影响。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！