胚胎

The argues about ethical aspects aroused by embryonic stem cells seem to be claming down, a new argue about embryonic stem cell-derived ga metes and its ethical impact comes out.

胚胎干细胞在伦理学界引发的争论尚未平息，胚胎干细胞源性配子的诞生又在更深层次对伦理学产生了新的冲击。

During the first 3 days of sequence culture, SOF and granulosa cell enhanced the development of porcine parthenogenetic embryos and the cleavage rate (P.05) and the number of beyond 4-cell embryos. During the following 3 days. NCSU-23 supplemented with FCS and POEC supported the embryo to morulae/blastocysts.

在序贯培养的前3d，SOF培养基和颗粒细胞对胚胎的发育有促进作用，分裂率(P.05)和突破4细胞阻滞的数目显著增加，在培养的后3d，添加胎牛血清的NCSU-23和输卵管上皮细胞能支持较多胚胎发育到桑囊胚，桑囊胚的发育率为(59.5±3.2)%(P.05)。

Confocal microscope detection experiments showed that, in morulae and blastocysts, with theincreasing concentration of BPA, embryo of cytochrome C release rate increased. This showedthat the cytochrome C involved in BPA-induced the embryos apoptosis process. This processis regulated by the mitochondrial pathway.

激光共聚焦显微镜检测细胞色素C结果显示：在桑椹胚期和囊胚期，随着BPA处理浓度的升高，释放细胞色素C的胚胎比率逐渐增加，说明细胞色素C参与了BPA诱导胚胎细胞凋亡的过程，这一过程是通过线粒体通路来调节的。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本试验以昆明小鼠1-细胞期胚胎为实验对象，以CZB培养液为基础液在不同发育阶段添加外源性ROS，培养至桑椹胚、早期囊胚、囊胚、扩张囊胚阶段，分别观察胚胎的胚细胞数、分裂指数的变化。

Methods\ The 6 5mol/L glycerol and 6% BSA were used as the vitrification solution for the cryopreservation of morulae and blastocysts embryos of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse.

采用 6 5mol L的甘油作为冷冻保护剂，采用二步法对CBA、NOD、C57BL 6J、ICR及CD1小鼠 3 5d的胚胎进行玻璃化冷冻，并比较了不同品系小鼠胚胎的复苏率及移植受孕率。

Expression of tert and hprt genes were detected simultaneous in all single-copy embryos developed at 4-C (65h-post hCG, 4/4), 8-C (75 h-post hCG, 4/4), morulae (93h-post hCG, 4/4) and blastocyst (118h-post hCG, 4/4) stages and the level of transcription increased.

结果表明，持家基因hprt在成熟卵母细胞受精前后，以及胚胎早期发育过程中均存在转录本。40%卵母细胞中存在的tert mRNA在受精后很快降解，检测不到；胚胎基因组在2-C早期开始转录tert mRNA，转录水平逐渐上升。

But because IVF is expensive and uncertain, most people want to create as many embryos as possible in one go. Extras are inevitable.

但是，由于IVF价格昂贵并具有不确定性，大多数人都希望一次能制造尽可能多的胚胎，因而就不可避免地有胚胎剩余下来。

Methods The mouse uterus of 1-3 days implantation embryos were separated, sheared and cultivated. Inner cell masses were dissociated and passaged in two days according to routine method of ES cells digesting and passaging.

分离小鼠胚胎着床初期1～3天（孕4.5～6.5天）小鼠子宫，剪碎后进行组织块培养，2天内分离内细胞团，以后按常规的胚胎干细胞培养及传代方法进行传代。

Geoffroy contended that embryonic development recapitulated transformations of organisms in past eras when the environment acted on embryos, and that animal structures were determined by a constant plan as demonstrated by homologies.

杰弗罗争辩说，胚胎发育重演了生物体在过去纪元的转化，在过去纪元里环境对胚胎起到了作用；而动物的结构是由一个不变的设计决定的，如同源相似所表明的那样。

Embryo sexing is one of the important parts of embryo engneering techniques, which has played an important role in livestock breeding, reproduction and prevention of genetic diseases.

胚胎性别鉴定是胚胎工程技术的重要组成部分之一，对家畜育种、生产和遗传疾病的防治均有非常重要的意义。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！