胚胎

Experiment Ⅴ: Mouse polyploid embryos were produced by electrofusing 2-cell blastomeres successionally.

实验六、通过2-细胞卵裂球交换制作2n／4n嵌合小鼠胚胎，对这种胚胎的发育能力进行了评价。

The two major events occurred in the beginning stage of reprogramming are: the Zygotic Gene Activation in 1-cell embryos and the formation of a Transcriptionally Repressive State during 2-cell stage.

其中最明显的两个变化是出现于一细胞胚胎中的合子基因表达激活与出现于二细胞胚胎中的转录抑制。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异；免疫组织化学方法检测EB1蛋白在两者中的表达情况，并用Western blot加以验证。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

结果 EB1基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值在人工流产绒毛核型分析正常的胚胎细胞中为0.01004±0.008223，在自然流产绒毛核型分析异常的胚胎细胞中为0.1509±0.0633。

OBJECTIVE: Persistence of all or a portion of the embryonic tract between the urinary bladder and the umbilicus results in a variety of urachal anomalies.

研究目的：脐尿管是胚胎时期连接膀胱和肚脐之间的通道，若是出生后此通道没有完全关闭，将因此胚胎时期的残迹衍伸出许多临床症状。

The soluble protein content decreased during embryonic development. Vitellin was a main component of soluble proteins. Besides, there were other soluble protein subunits such as 243 ku, 181 ku, 67 ku, 54 ku and 31 ku proteins.

可溶蛋白的含量在胚胎发育过程中逐渐降低；在成熟卵细胞和胚胎期，可溶蛋白在组成上以89ku和100ku的卵黄磷蛋白(Vitellin， Vn)为主，同时还存在243ku、181ku、67ku、54ku和31ku等其他一些蛋白亚基。40ku蛋白亚基仅出现在成熟卵细胞中，推测可能参与执行了特定的生殖功能。

Tolerance threshold of 2-cell mouse embryos to ethanol was around 1.5% when these embryos were cultured in Whitten's medium with 0, 0.1%,0.5%,1.0%, 1.5%, 2.0%, 3.0%, 5.0% and 10.0% ethanol, respectively.

用含不同浓度乙醇的Whitten氏培养液对小鼠2细胞、4细胞、8细胞和桑椹期胚胎分别进行体外培养，研究了乙醇对小鼠不同发育时期胚胎体外发育的影响。

The development and maturity of zygotic embryos required a higher level of acyltransferase.Our results showed that ERT and SCL were unique during somatic embryo development,the rest of transcription factors were not remarkable difference with the expression level between two types of embryo development process.

合子胚的发育和成熟要求更高水平的酰基转移酶；除ERT和SCL转录因子是体细胞胚胎发育所特有的，其余转录因子的表达水平在两种类型的胚胎发育过程中没有明显特异之处。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示，在卵母细胞和8细胞期胚胎，基因calm3和trm112l的表达量无显著性差异，而在囊胚期的表达量与前两个时期的表达量存在显著性差异，推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控，提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

During somatic embryogenesis and zygotic embryos development,the start-up and morphogenesis of embryos are regulated by a variety of factors.In particular,somatic embryogenesis is a temporal and special regulated developmental process.

在体细胞胚胎发生和合子胚发育的过程中，无论是胚胎发生的启动还是胚状体的形态建成都受到多种因子的调控，体细胞胚发生是一个由多种基因参与的复杂时空调节的发育途径。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！