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To explore the relations of the strains of embryo and pseudopregnant recipient to live birth rate of embryo transfer and the feasibility of mixed transfer of different embryo strains.

探讨不同受体鼠和供体鼠品系对胚胎移植成功率的影响以及不同品系胚胎混合移植的可行性。

Objective : To measure the differences of total T cell percentages of peripheral blood, peripheral immune organs and central immune organs of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time, and to analyze the effects of these differences on interspecific embryo implantation.

目的:研究种间胚胎植入期母体外周血、外周免疫器官、中枢免疫器官中总T细胞的百分比变化,并探讨这种变化对种间胚胎植入的影响。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法:利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变,使编码子249由精氨酸变成丝氨酸,然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中,将注射过的囊胚植入假孕的雌性小鼠子宫,到第14d取小鼠胚胎纤维母细胞,用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞,经测序证实细胞含有由249Arg到Ser的突变。

Objective: By investigate the difference of nitric oxide contents in adult and fetal rabbit wound healing, it was expected to probe the potential reasons of fetal scarless wound healing.

目的:分析胚胎无瘢痕愈合的潜在原因,研究NO在成人型和胚胎型愈合过程中的差别。

Objective: By investigate the difference of nitric oxide contents in adult and fetal rabbit wound healing, it was expected to probe the potential reasons of fetal scarless wound healing.

导读:摘要目的:分析胚胎无瘢痕愈合的潜在原因,研究NO在成人型和胚胎型愈合过程中的差别。

Objective To detremine whether fetal cartilage shares the unique scarless healing properties of fetal skin. Methods Full thickness in cisional ear wounds were created and sutured in fetal rabbits at 23 days' gestation(full term, 31 days')and in adult control rabbits.

摘 要:目的研究胚胎软骨在创伤愈合过程中是否也具有无瘢痕愈合的特点方法将孕23天(孕期 31天)的胚胎兔耳全层切开,缝合后送回子宫内。

Except on the testis tissues,the expression of ZFY gene were found on the kidney tissues of human embryo or rat, brain vesicles〔rat embryo 13 days〕,and sclerotome cells〔rat embryo 13 days〕 It is possible that because kidney and genital system come from same origin,ZFY gene expressed on the kidney tissues too, the expressive function of ZFY gene on the embryonic brain vesicles and sclerotome cells is not yet clear.

除了在睾丸组织上表达外,ZFY基因还在肾脏组织及鼠胚脑泡(胚胎13天),生骨节细胞(胚胎13天)上表达。在肾组织上表达可能是肾脏与生殖系统起源相同有关。在胚脑泡及生骨节细胞上表达其作用不清楚。

Analyzed pregnancy rate of sexed embryo on embryo character , recipient character and their correlativity .

本研究在胚胎性状,受体和两者之间的相互关系方面分析了性别鉴定胚胎的移植妊娠率,结果如下

Under our conditions, detection rate of embryonic DNA were zero with 2 cells, 62.5% with 4 cells, 90% with 8 cells and 100% with 16 cells. A total of 23 embryos that had been biopsied and sexed were transferred into the donor...

对 2 3枚经过性别鉴定后的胚胎进行移植,获得 5头纯种试管牛,产犊率为 2 1 7%,与正常的胚胎移植结果 2 7 3%( 3 1 1 )无显著差异,预测性比为 1 0 0 %符合。

We discuss some microRNA that are specificly expressed in embryonic stem cell and their regulating effect on self-renewal and differentiation .

主要讨论一些在胚胎干细胞中特异性表达的microRNA,以及这些microRNA 对胚胎干细胞自我更新和未分化状态的维持和继续分化增殖的调控作用。

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