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Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的:研究大鼠胚胎脊髓移植后能否影响,c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复,方法;将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组,每个时相点每组6只动物,术只1,3,7,14和28天,应用行为学和电生理检查观察大鼠功能恢复情况,应用免疫细胞化学方法,c-Jun免疫反应的表达,采用计算机图像分析技术,进行定量分析。

"Evolution" was first used in Preformationism and then in Epigenesis, respectively, to describe the unfolding of preexisted organic structure and the recapitulation of the forms of lower animals by the embryo of higher animals. Then the meaning of species change was added to it in the beginning of the nineteenth century.

&Evolution&一词先后被用在胚胎学的预成论和后成论中,分别用来描述预先存在的有机体结构的展开以及高等动物胚胎对低等动物形式的重演。19世纪初它又被添加上了物种改变的含义。

"Evolution" was first used in Preformationism and then in Epigenesis, respectively, to describe the unfolding of preexisted organic structure and the recapitulation of the forms of lower animals by the embryo of higher animals. The n the meaning of species change was added to it in the beginning of the nineteenth century.

&Evolution&一词先后被用在胚胎学的预成论和后成论中,分别用来描述预先存在的有机体结构的展开以及高等动物胚胎对低等动物形式的重演。19世纪初它又被添加上了物种改变的含义。

On the 8th and 9th day of pregnance, the mice received one time of general irradiation of 60Co and γray respectively, the dosage of irradiation was 1.0 Gy;on the 10th day ,the mice were put in hotboxes keeping their rectal temperature at 37±0.5℃, 41±0.5℃ and 42±℃0.5℃ persisting for 3,4 and 5 mins, respectively.

目的 探讨高温联合辐射致胚胎神经发育与早期胚胎脑蛋白质和HSPs表达的关系。方法将130只孕鼠按随机抽签分为13组,每组10只。

Among these significant advances has been the ability to alter the genetic constitution of eggs by pronuclear and nuclear transfer as well as by the introduction of specific cloned genes into eggs and embryos.

近年来,应用于研究哺乳动物卵子和胚胎新的实验方法已有重大发展,为深入研究哺乳动物胚胎学提供了前所未有的重要手段。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析,结果显示:(1)小鼠卵母细胞的乙醇激活结果表明,随着乙醇浓度的升高和作用时间的延长,卵母细胞激活率和发育率都显著提高,但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加,7%乙醇作用卵母细胞7min 为最佳激活条件。

A corresponding portion of the egg of other animals, consisting of protein and fat which serve as the primary source of nourishment for the early embryo and protoplasmic substances from which the embryo develops.

卵黄其它动物蛋内的相应部分,由蛋白质和脂肪组成,用作早期胚胎的主要营养来源和胚胎由此发育的原生物质

A corresponding portion of the egg of other animals, consisting of protein and fat which serve as the primary source of nourishment for the early embryo and protoplasmic substances from which the embryo develops.

卵黄其它动物蛋内的相应部分,由蛋白质和脂肪组成,用作早期胚胎的主要营养来源和胚胎由此发育的原生物质主要实施生物质气化集中供气系统工程和大型沼气能环工程。

No embryos developed to term after transfer of the pronuclei stage embryos to oviducts of pseudopregnant mice.

将体外受精得到的重组卵来源的双原核胚胎进行输卵管移植,没有胚胎发育到期。

AIM: To explore the relations of the strains of embryo and pseudopregnant recipient to live birth rate of embryo transfer and the feasibility of mixed transfer of different embryo strains.

目的: 探讨不同受体鼠和供体鼠品系对胚胎移植成功率的影响以及不同品系胚胎混合移植的可行性。

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