胚胎

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

Therefore, a experimentent with three exposure types (early cleavage exposure, 0hpf; gastrula exposure, 8hpf and 0hpf exposure but stop after 8 hours, 8h stop) were designed to investigate the toxicity and bioaccumulation of bisphenol A in zebrafish embryo in different stages.

为研究胚胎在不同发育阶段对外源化合物的毒性敏感差异，以0hpf、8hpf和8h终止3种暴露方式进行染毒，探讨斑马鱼胚胎在不同暴露时段对双酚A毒性和生物蓄积特征。

Therefore, a experimentent with three exposure types (early cleavage exposure, 0hpf; gastrula exposure, 8hpf and 0hpf exposure but stop after 8 hours, 8h stop) were designed to investigate the toxicity and bioaccumulation of bisphenol A in zebrafish embryo in different stages.

为研究胚胎在不同发育阶段对外源化合物的毒性敏感差异，以0hpf、8hpf和8h终止3种暴露方式进行染毒，探讨斑马鱼胚胎在不同暴露时段对双酚A。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

The embryological studies of strawberry showed that the ovule of strawberry were hemitropous, the ovule had the obvious hypostace structure, the type of its embryo sac was Polygonum, the embryo sac was long and narrow, the type of development of embryo was the Geum type of the Asterad type, the endosperm was Nuclear type and the somatic embryo sacs happened The integral transparency technique combined with the technique of microdissection was used in this experiment The combination was an innovation in the techniques of section.

对草莓胚胎学的研究结果表明：草莓胚珠横生、胚珠具明显的承珠盘结构、胚囊发育为蓼型、胚囊狭长、胚胎发育为紫菀型水杨梅类型、胚乳核型、并有体细胞胚囊的产生。试验中采用了多种制片方法，其中将整体透明技术和显微解剖技术相结合的方法是在制片技术上的一次创新。

Objective: To optimize the culture system of hEGcs in vitro, select the hEGcs from subculture to transplant directly to the rat infarcted myocardium and observe the therapeutic mechanism of their heterotransplantation in the treatment for the rat myocardial infarction.

目的：优化人胚胎生殖细胞的体外培养体系，并将生长状态良好的传代的人EG细胞移植用于大鼠心肌梗死模型的治疗，探讨人胚胎生殖细胞进行异种移植对大鼠心肌梗死的治疗机制。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

To investigate the interaction between heat shock protein 70(HSP70) and alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402,the expression and localization of HSPT0 and AFP in human hepatocellular carcinoma cell line BEL-7402 were determined by immunocytochemistry and indirected immunofluorescence cytochemicai staining.

甲胎蛋白是由胚胎肝细胞、卵黄囊和胚胎消化道微量合成的主要血清蛋白[1]。

Objective: Identify the human embryonic germ cells by observing the expression of its specific surface markers SSEA-1、SSEA-3. Methods: Human embryonic genital ridges, dorsal mesenteries(5-9 weeks post-fertilization) were cultured without any cytokines, while the fibroblasts derived from the isogenous embryo were used as the feeder layer.

目的：组织块培养法培养人胚胎生殖干细胞，通过检测细胞所表达的阶段特异性胚胎抗原1 和3(stage-specific embryonic antigen-1、3 ，SSEA-1、SSEA-3)，对未分化的人EG 细胞进行特异性的生物学鉴定。

Furthermore, we investigated the dynamic characteristics of lamin B by immunofluorescence during the nuclear envelope reconstruction of PM-embryos.

本研究表明：在去除抑制剂3h后未能形成核膜的胚胎进一步卵裂，而形成核膜的PM胚胎培养24h未见进一步发育。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。