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METHODS: Embryos were transferred to oviducts of 106 recipients by the traditional method (tearing the bursa open over the infundibulum of oviduct) or the improved method (puncturing a hole in bursa with embryo transfer pipette and blowing embrys into oviduct through the hole).

目的: 通过将传统的撕破囊膜进行小鼠胚胎移植的方法改为囊膜穿刺,以期提高胚胎移植的成功率。

Early embryos dissected from host larvae hemocoel grow bigger in size and proliferate by the invagination of extroembryonic membrane, but they can not develop into the individual stage.

从寄生初期寄主幼虫体内得到的初级胚胎可以在体外培养条件下长大并持续通过胚外膜凹陷进行增殖,但无法进入个体发育阶段,胚胎最后黑化死亡。

In common with many other kangaroos, the brush-tailed rat kangaroo mates shortly after giving birth and can keep embryos in embryonic diapause until they are needed.

同许多其他袋鼠,刷尾鼠袋鼠队友生完孩子后不久,可以让胚胎胚胎滞育,直到他们是必要的。

Kirov et al. and Llevadot et al. successively found a new member of the HIRA family, DHIRA from D. melanogaster. Northern analysis showed that hybridization is strong in female adults, unfertilized eggs and 0-3-h-old embryos, then diminishes, but is still detectable.

Northern分析表明,在雌性成体、未受精卵和0-3时龄的胚胎中,dHira的转录本很高,之后急剧下降,说明它们在早期胚胎发育过程中可能起了重要的作用,但其具体功能还未见报道。

Based on the morphological observation,the activities of SOD were measured during embryonic development of longanDimocarpus longan Lour.

在对红核子龙眼胚胎发育过程进行形态学观察的基础上,对龙眼胚胎发育过程中SOD活性变化进行测定。

After two hours of the mice leaveing warming boxes, 5 mice randomly selected from each group per timesection were executed by cervical vertebra luxation.

移出温箱2h后,每组每时段随机取5只脱颈法处死,采用Western dot blot 和lowry方法定量分析胚胎脑HSP70和蛋白质含量与胚胎神经系统畸形。

After two hours of the mice leaveing warming boxes, 5 mice randomly selected from each group per timesection were executed by cervical vertebra luxation. The HSP70 of embryonic brain, protein content and deformations of embryonic nervous systems were quantitatively analyzed with the Western dot blot and Lowry method.

移出温箱2h后,每组每时段随机取5只脱颈法处死,采用Western dot blot 和lowry方法定量分析胚胎脑HSP70和蛋白质含量与胚胎神经系统畸形。

Then the mouse embryos at the stage of 2-cell, 4-cell, 8-cell and morula were cultured in Whitten's medium with 1.0% and 3.0% ethanol to see the sensitivity of mouse embryos to ethanol.

首先利用含0、0.1%、0.5%、1.0%、1.5%、2.0%、3.0%、5.0%和10.0%乙醇的Whitten氏培养液对2细胞胚胎进行培养,发现小鼠2细胞胚胎对培养液中乙醇浓度的耐受极限在1.5%左右。

Peg3 showed completely unmethylated in two cell embryos, and was strongly de novo methylated in morula stages.

结果显示,在正常发育胚胎中,Peg3甲基化印迹在小鼠2细胞胚胎之前就已经被擦除,直到桑椹胚时才被重新建立起来。

There are significantly differences on H_2O_2 levels between embryos in vivo and cultured in vitro on the early 2-cell stage and late 2-cell stage, but there are no significantly differences among embryos those on the 4-cell stage, morula and blastula using SPSS statistics software.

我们测量的结果是体内2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是52.92,50.77,48.84,39.96和30.36;体外2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是74.85,82.62,82.98,81.28和54.09。

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