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Project Content and scale: 1. To construct 588m2 embryo production center, purchase 368sets of equipments and instruments. 2. To build and perfect the breeding farm of milk cow of fine breed, enlarge the cattle pen and the sports ground, repair the milking room and other auxiliaries. To introduce 400 bulls kept for covering.

项目建设内容及规模:1、建设588米2的胚胎生产中心,购置仪器设备368台套;2、建设完善良种奶牛繁育场,改扩建牛舍、运动场,修建挤奶厅、兽医室及其他附属设施,引进纯种供体牛400头,利用胚胎移植技术繁殖牛群使存栏达1000头。

At the same time,there were not marked difference in the numbers of biastomere and mitotic index of control group and all treatment groups, suggesting that 2 -celt embryos were not only most sensitive to exogenous H2O2 but also rasiest to occur to development block.

但是经H2O2处理之后,度过2-细胞期发育阻滞的胚胎能在以后的发育中发生补偿性生长,其胚细胞数和分裂指数都和体外正常发育的胚胎没有差异。

①The development of goat embryonic epidermis, which differentiates exquisitely during the embryo phase, is priority to that of corium.

①山羊胚胎表皮的发生优先于真皮的发生,在胚胎时期处于剧烈的分化阶段。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体,分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植,构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应,并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法,探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响,旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

On days 8 and 9, a low level of COX-1 immunostaining was observed in the whole decidua.

胚胎着床后,在着床胚胎周围的腔上皮下基质细胞中可检测到COX-2 mRNA和蛋白的表达。

The presence of a large population of apoptotic cells in chorionic villi and decidua might contribute to the morphological changes of these tissues, and also the development of the fetus.

细胞凋亡在胚胎的发育过程中起着重要作用,自然流产绒毛和蜕膜组织中大量的细胞凋亡是其形态学退变的重要原因,并进而阻碍了胚胎的发育。

During embryonic development, many animals form a number of temporary structures along their anterior-posterior axis-somites. Cells in somites differentiate into sclerotome, which gives rise to vertebral column, dermatome, which gives rise to dermis, and myotome, which gives rise to skeletal muscle.

脊椎动物在胚胎发育的过程中沿身体前后轴形成一定数目的暂时性结构-体节,随着胚胎的继续发育每个体节分化成为生骨区、生皮区和生肌区,继而生成各种组织。

At approximately 24 hours post-fertilization, fez expression is mostly confined to the telencephalon and the anterior-ventral region of the diencephalon.

受精后24小时的斑马鱼胚胎中,Fez的表达范围主要分布在胚胎的端脑和间脑的腹侧前端。

The development of embryos from diploid somatic cells in embryo culture.

胚胎培养过程中,二倍体细胞发育为胚胎的过程。

Currently ESCs have been used in the therapy of diabetes mellitus, Parkinson's disease and cardiovascular injury. Even though many problems of technique and ethics should be done, we believe that ESC therapy will be a promising method and used in clinical practice if we make clear the mechanism of ESC directional differentiation, establish an optimal culture system, and solve the problem of oncogenicity.

目前胚胎干细胞已经应用在治疗糖尿病、帕金森及心血管损伤等各种疾病中,虽然有很多技术问题和社会伦理问题需要解决与克服,相信随着胚胎干细胞定向分化机制的逐渐完善,优化培养体系的建立,致瘤性的解决,该细胞疗法作为一种新的治疗方式,将适用于临床使用。

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