胚胎

Between P14 and P21,CIAPIN1 immunoreaction in the brain,heart and liverbecame much lower. However,between P21 and P28,CIAPIN1 immunoreactionin the heart,brain,liver and skeletal muscle became much lower,while with thekidney development,CIAPIN1 immunoreaction in the kidney became higher. Invarious tissues from adult mouse,CIAPIN1 immunoreaction could be seen incardiac muscle cell,brain,hepatocyte,epithelium of renal tubule,skeletal muscle,lung tissue,gastric mucosa and gland,acinus lienalis.2. Distribution of CIAPIN1 in normal fetal and adult human tissuesTo reveal the possible physiological role of CIAPIN1,we examined theexpression and distribution of CIAPIN1 in fetal and adult human tissues usingimmunohistochemistry. We found that CIAPIN1 was ubiquitously distributed infetal and adult tissues,and was localized in both the cytoplasm and the nucleus.

然而，在3个月大的成年鼠中，CIAPIN1阳性反应物在心、脑、肝和肾小管中的表达强度要低于P28小鼠；但CIAPIN1阳性反应物在成年鼠骨骼肌中较P28小鼠高。2、CIAPIN1蛋白在人5个月胚胎及成人多器官组织内的表达在人5月胚胎多器官组织中，CIAPIN1阳性反应物见于心脏、胆囊单层柱状上皮和粘膜、结肠粘膜、小肠粘膜和绒毛、肝脏、直肠腺体、胃粘膜、肾上腺束状带、甲状腺滤泡、脾索、胸腺小叶间隔、皮肤真皮层和汗腺、睾丸白膜和间质、脑组织内神经元和神经胶质、肺小支气管和肺泡、骨骼肌、肾脏皮髓质和肾小管、子宫内膜、胰腺腺泡和胰岛、卵巢、输卵管粘膜等绝大多数组织细胞。

Our survey seriously undermines the credibility of Haeckel's drawings, which depicts not a conserved stage for vertebrates, but a stylised amniote embryo.

我们的研究严重削弱了海克尔图形的可信性，海克尔图形与其说是显示脊椎动物胚胎发育过程中一个相似时期，不如说是按固定程式设计的胚胎。

The yolk proteins were collected through the anatomization of the maiden eggs and the eggs in different embryo development stages. The yolk proteins were separated by two-dimensional electrophoresis and followed the image analysis. Over 203 protein spots were found to exist in the yolk protein of silkworm eggs. Some different patterns of yolk protein spots were found in silkworm of various varieties. While, little variation of yolk proteins was detected during different development stages of any silkworm variety.

采用直接解剖蚕卵获取卵黄蛋白，结合蛋白质双向电泳和图像分析，对家蚕处女蛾卵及不同胚胎发育阶段蚕卵的卵黄蛋白质进行了研究，发现家蚕卵黄蛋白有 203 个蛋白质斑点；不同家蚕品种之间卵黄蛋白的组成存在一些差异；同一品种不同发育时期胚胎卵黄蛋白的组成基本保持不变。

After confluence, there is no clear margin between cells and only nuclear apophysis could be observed. Cell monolayer was smooth like quiet lake surface.

自制的胚胎冷冻液含有三种不同的冷冻保护剂(包括渗透性和非渗透性保护剂)，冷冻胚胎解冻后可用率在95％左右，比常用的DMSO冷冻液更适宜于这三种细胞的冷冻保存。

During early pregnancy, the endometrial cells can form atemporal permeability barrier around the embryo to protect it from the maternal harmfulenvironment.

在妊娠早期过程中，为了保护胚胎免受母体不利环境的侵害，子宫内膜细胞在胚胎的周围形成了一个短暂的渗透屏障。

The principle fields of the Institute's activity are related to molecular genetics, cytogenetics, immunogenetics, embryology, biochemistry, the physiology of animal stress and ethology, embryo biotechnology and biotechnics, introductions of the results of basic investigations into animal improvement programmes, determination of animal genotypes and their adaptation to environmental conditions and production demands.

该所的主要活动领域是关于分子遗传学、细胞遗传学、免疫遗传学、胚胎学、生物化学、动物胁迫和动物行为方面的生理学、胚胎生物技术和应用生物学、改善动物的项目基础研究结果的介绍、动物基因型的决定及其对环境条件的适应与产品要求等方面的内容。

Objective To investigate the effect of different splitting solutions and decompaction treatment on the bisection of mouse embryos at morulae and blastocysts stage and their subsequent transfer after cultured.

目的 研究不同分割液和分割前胚胎去致密化与否对昆明白系小鼠的桑椹胚和囊胚的徒手分割以及分割后胚胎移植的影响。

There were 8 embryos single blastomere amplified, and the amplification success rate was 72. 7%(8/11); 3 embryos' not amplified; 2 embryos'showed preferential amplification , and the PA rate was 25%(2/8); and the 7 tubes of blank with washing cell solution were not contaminated. We transferred 3 unaffected embryos to the uterus. But unfortunately, blood hCG was negative after 2 weeks of transferring indicating no pregnancy.

共8枚胚胎的单卵裂球得到扩增，扩增成功率为72.7%（8/11），3枚未得到扩增，2枚发生了偏性扩增(preferentialamplification，PA)，PA的发生率为25%（2/8），细胞洗脱液空白对照7管均无污染发生。3枚诊断为正常的胚胎被移植入宫内，不幸的是，移植后2周，血hCG阴性，证明未妊娠。

Pregnancy is a complicated process, it contains the early implantation of blastocyst and the embryonic development.

妊娠是一个复杂的过程，包括早期的胚胎着床及其之后的胚胎发育。

Human embryonic stem cells are pluripotent cells derived from inner cell mass of blastocyst at early stage of human embryo.

人胚胎干细胞(human embryonic stem cells，hES cells)是一种从人类早期胚胎的内细胞团中分离出来的多能性干细胞。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！