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These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位,同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值,本研究将pEGFP-N1基因导入小鼠ES-D3细胞系,得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP,通过对昆明小鼠的囊胚腔注射,获得了4只表达绿色荧光蛋白的嵌合体小鼠。

Nes and tissues, the embryo, and all postembryonic tissues and organs.

全能细胞具有分化为特定的胚胎外膜和组织、胚胎以及所有胚胎

During the process of nuclear reprogramming, the zygotes of the normal embryos and the donor nuclei of thecloned embryos cease their unique repertoire of gene expression and reset their gene expression to the totipo-tent status, and then the normal and cloned embryos redifferentiate from the totipotent status to variousdifferentiated states for tissue generation or organogenesis.

通过细胞核重编程,首先,受精卵和克隆胚胎的供体核停止其特有的基因表达程序,恢复为全能状态的基因表达程序;然后,受精胚胎和克隆胚胎的细胞再从全能状态重新进入分化状态,最终形成各种组织和器官。

The use of Frozen Gender Selected Embryos The gender selected embryos means that the embryos are sexed following collection from the donor cow and only the females are implanted.

冷冻性的使用选择了胚胎性选择了胚胎胚胎被性别来自捐赠人母牛的下列收集的方法,而且只有女性被深植。

Thyroglossal duct cysts are the most common cervical cysts and are usually considered to be a benign embryonic malformation where the thyroglossal duct fails to obliterate. Parathyroid cysts are a rare disease and may also result from malformation of the remnants of the third pharyngeal pouch.

甲状舌骨囊肿是最常见的颈部囊肿,一般认为是胚胎发育过程中,甲状舌管没有关闭造成的良性胚胎畸型;副甲状腺囊肿则是一种罕见疾病,可能起因於胚胎发育时第三咽囊的残存物。

Results:Low frequency ultrasound of 9w/cm2,220KHz irridiating the embryos could not cause significant hyperthemia.Three kinds of electrocardigram were detected after irridiating embryos:tachycardia for some minutes,disturbing rhythm of the heart and unrecovered slow rhythm of heart.Unrecovered slow rhythm of heart sound appeared in 77.5%embryonic electrocardiogram when irridiating for ten minutes without temperature rise found.

结果:应用9W/cm2、220KHz的超声辐照时间在10分钟内,均不会导致胚胎温度的异常升高;超声辐照后胚胎心电出现三种变化形式即心率加快后恢复正常、心律紊乱和不可逆转的心率变慢,其中辐照10分钟,有77.5%胚胎出现不可逆心电变慢且无异常温度升高。

This paper was mainly working on the biochemical changes (water, carbohydrate, lipid, fatty acid, protein, amino acid) during embryonic development of Cherax quadricarinatus. At the same time, we especially worked on the yolk protein-vitellin in two aspects: 1. Isolation, purification and characterization of vitellin from the ovary of female Cherax quadricarinatus.

本文对红螯螯虾胚胎发育过程中的主要生化物质(水分、碳水化合物、脂类、脂肪酸、蛋白质、氨基酸)进行了测定,特别是对胚胎发育过程中主要的营养物质——卵黄磷蛋白的结构和含量变化做了细致的研究,同时讨论了这些物质的变化与红螯螯虾胚胎发育形态发生的相关性。

Although Pygo expressed ubiquitously in the embryo, it expressed at relatively high level in pre-blastoderm embryos which indicated a high degree of maternally provided message, followed by a low level of ubiquitous zygotic expression.

结果表明,虽然pygo普遍表达于整个胚胎中,并且在果蝇胚胎发生全过程都有,但Pygo在前囊胚层(pre-blastoderm)中的表达水平相对较高,这说明胚胎发生过程中来自母方的贡献较高。

Quality of this kind of silage is good, the loss is used smally, more generally, but cost is relative taller; 3 tower lay aside: The tower is made with armor plate, volume is very large, ensiling quality is good, but cost is higher, general ox field is not used.

这种青贮质量好,损失小、较普遍被采用,但造价相对较高;3)塔贮:塔用钢板制成,容积很大,青贮质量好,但造价更高,一般牛场不采用。2人工授精及胚胎移植牛的人工授精在阿尔伯达已有40多年的历史,在胚胎移植方面也处于世界领先地位一将胚胎移植用于商业即是从阿尔伯达开始的。。。

Thirdly, we incubated the gene/ liposome complex with sperm, then artificially inseminated, and 53 of 357 embryos had fluorescence.

PCR检测得到的胚胎,发现表达绿色荧光蛋白的胚胎其PCR反应阳性率为100%,说明外源基因在胚胎发育早期可以表达。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。