胚细胞

The quantities of starch grains in the two"cells"in micropyle part of embryosac gradually decrease and become unclear.With the further development of embryosac the starch grains in the central cell and antipodal cell disappear in succession,however,the egg cell does not.

在准极核移向胚囊中部前后，不同的功能性细胞有不同的表现，珠孔端的两个"细胞"淀粉粒数量逐渐减少并变得不明显，随着胚囊的进一步发育，其中中央细胞和反足细胞内的淀粉粒相继消失，但卵细胞内却一直保存着。

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同，滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%，显著高于昆明鼠(47.6%)；自发激活率分别为13.3%和46.0%，C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%，显著低于SrCl_2孤雌激活率(96.4%)；自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%)，但囊胚率(18.7%)较低，同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%，差异不显著。

The quantities of starch grains in the two"cells"in micropyle part of embryosac gradually decrease and become unclear.With the further development of embryosac the starch grains in the central cell and antipodal cell disappear in succession,however,the egg cell does not.

在准极核移向胚囊中部前后，不同的功能性细胞有不同的表现，珠孔端的两个&细胞&淀粉粒数量逐渐减少并变得不明显，随着胚囊的进一步发育，其中中央细胞和反足细胞内的淀粉粒相继消失，但卵细胞内却一直保存着。

These experiments demonstrated that hyperglycemic conditions in vitro can inhibit the growth and development of the embryo cells resulting in the down-regulated expression of igf-1, and IGF-1 could inhibit the apoptosis of blastocyst and be benefit for the growth of blastocyst.

因此，高血糖能抑制胚泡细胞的生长发育，导致igf-1的表达下调，而IGF-1能抑制胚泡细胞的凋亡，有利于胚泡细胞的生长发育国。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

During embryogenesis, CaM mRNA was expressedstronger in the endosperm cells than in the proembryo cells at earlier stage but it wasreversed at embryo differentiation stage.

胚胎发育早期，钙调素mRNA在胚乳细胞中的表达比原胚中强，而后期则在分化胚中比胚乳细胞中强。

Then one ovule degenerated, the other which contained zygote kept on developing. Embryo undergone proembryo and globular embyo stage in early part of June. Heart stage embryo developed into torpedo stage, and meanwhile nuclear endosperm formed cellwalls in later June. Birch Fruit matured in July..

此后，一个胚珠退化，另一个含有受精卵的胚珠继续发育。6月上旬，白桦胚正经历原胚球形胚阶段。6月下旬核型胚乳细胞化，心形胚发育为鱼雷胚。7月，白桦果实成熟。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Confocal microscope detection experiments showed that, in morulae and blastocysts, with theincreasing concentration of BPA, embryo of cytochrome C release rate increased. This showedthat the cytochrome C involved in BPA-induced the embryos apoptosis process. This processis regulated by the mitochondrial pathway.

激光共聚焦显微镜检测细胞色素C结果显示：在桑椹胚期和囊胚期，随着BPA处理浓度的升高，释放细胞色素C的胚胎比率逐渐增加，说明细胞色素C参与了BPA诱导胚胎细胞凋亡的过程，这一过程是通过线粒体通路来调节的。

Somatic embryos can be produced both from the surface and the inner of the calli. Embryogenic cell has normal shape, dense cytoplasm , clear nucleolus, hearty metabolize; Plantlet regenerations were developed from global embryo, heart-shaped embryo, torpedo-shaped embryo and cotyledon embryo, in which the V-shaped vessel vascular bundles and shoot tip meristem and root tip meristem were observed.

胚性细胞多为椭圆形，细胞质浓厚，核仁明显，代谢旺盛；体细胞胚经过球形胚、心形胚、鱼雷形胚和子叶胚而发育成再生植株，子叶期体细胞胚可观察到明显的V型维管束、茎尖分生组织以及根尖分生组织。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。