胚细胞

Objective To investigate the expression of inflammtory factor genes change induced by sodium sulfite.

目的 探讨亚硫酸钠(NaSO3)对人胚肾293(HEK293)细胞炎性因子表达的影响。

Suspensor A temporary stalklike row of cells, found in angiosperms, that differentiates from the proembryo by mitosis and pushes the embryo into the nutritive endosperm after fertilization.

胚柄：被子植物中临时由一串细胞排列而成的茎样结构。

It takes about 0.5 h for a pollen tube from germination to arrive at the micropyle. About 0.5 h after pollination, the pollen tube releases two sperms into one synergid. At 0.5-2.5 h after pollination, the egg cell fuses with one sperm to form a zygote. About 10.0 h after pollination, the division of the zygote begins. The duration of the zygote lasts about 2.5-10.0 h. However, one sperm nucleus fuses with two polar nuclei at 1.0-3.0 h after pollination, and 5.0 h after pollination the primary endosperm nucleus begins to divide.

水稻受精过程经历的时间表如下：授粉后，花粉在柱头萌发；花粉萌发至花粉管进入珠孔大约需要0.5小时；授粉后0.5小时左右，花粉管进入一个助细胞，释放精子；授粉后0.5-2.5小时，精卵融合形成合子；授粉后约10.0小时，合子第1次分裂，合子期为授粉后2.5-10.0小时；授粉后1.0-3.0小时，精核与两极核融合；授粉后约5.0小时，初生胚乳核分裂。

Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

采用原代培养和传代培养法，对1头毛冠鹿的胚肺组织细胞进行观察，发现一种新的核型，其二倍体染色体数目为2n=48，核型公式为2M+2ST+42T+XX，出现1对大的末端着丝粒染色体，C-带显示该染色体与已报道的相关染色体同源，首次提出毛冠鹿B染色体多态，对其传递机制进行了探讨。

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体，以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

Monosomic 3, monosomic 5, monosomic 10, trisomic 4 and trisomic 5 as experiment materials, their meiosis behavior of pollen mother cell, pollen characteristics, physiological and biochemical characteristics of anther, section observation of pollen development and the structure of embryo sac were studied, and the effects on the seed set and transmission rate were analyzed.

本文以大白菜同源四倍体小孢子培养产生的且已鉴定为3号单体、5号单体、10号单体、4号初级三体、5号初级三体的非整倍体株系及二倍体株系等为试材，对各材料的结籽率、雄配子传递率进行了统计，并对花粉母细胞减数分裂行为、花粉特性、花粉发育和胚囊结构以及花药生理生化特性等方面进行了观察研究，进而分析了其对大白菜单体n-1雄配子和三体n+1雄配子传递率的影响。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Methods: 63 Patients were randomly divided into the treatment group (31 cases) and the control group (32 cases). All patients of the two groups accepted FOLFOX 4 chemotherapy, while those in the treatment group treated with Shiji Decoction in addition. Effective rate, Quality of lief, CEA, state of cellular immunity were observed after two-cycle treatment.

将63例大肠癌患者随机分为研究组和对照组，两组均予以相同标准化疗方案进行治疗，研究组加用中药十济汤口服；治疗2个疗程后比较两组肿瘤治疗的有效率、患者生存质量、癌胚抗原、细胞免疫状态等指标。

The results were as follows: 1.Inoculated on the induction medium at the late uninucleate stage,the microspores could initiate embryogenesis by first divisions in both symmetric and asymmetric patterns,but the former was predominant.

通过观察玉米小孢子胚胎发生的细胞形态学特征，发现：离体培养条件下单核后期的花粉可以通过均等分裂和不均等分裂两条途径形成胚状体，与前人的研究结果一致。

Investigation of DNA replication levels in primed seeds with flow cytometric determination showed that the 6C:3C ratio in embryonic root tip cells was higher than the unprimed seeds at all stages of seed imbibition.

流式细胞分析仪对引发种子DNA复制水平调查结果表明，SMP处理的种子在种子吸胀的各个阶段，胚根尖中6C与3C的比值都高于对照种子。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。