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There were all kinds of tissue cells in the teratomas acquired from in-vitro differentiation, such as, neuro epithelium, skeletal muscle, respiratory epithelium. This further proved the isolated mouse ES cells could differentiate into three germ layers of tissue types.

体内分化实验所获得的畸胎瘤中存在各种组织细胞,如一些神经上皮样组织;横纹肌;呼吸上皮样组织等,这进一步证明所分离获得的小鼠ES细胞具有三个胚层的分化能力。

With the assistance of molecular probes for reactive oxygen species, the production and localization of ROS derived from embryo, starch and aleurone layer of maize seeds with different ages were investigated under a laser scanning confocal microscope and a luminescence spectrometer.

摘 要:借助活性氧发光分子探针,利用荧光光谱和激光共聚焦扫描显微技术,在不同年份的玉米种子吸胀早期,对其胚、淀粉和糊粉层细胞活性氧生成进行了定量和亚细胞定位分析。

The proliferation of osteoblast was measured by thiazolyl blue,activity of alkaline-phosphotase was observed with Golden s method and alizarin red stain was used to measure the mineral nodes of osteoblasts.

结果 0 10 g·L-1的麦胚提取物可以促进体外培养大鼠成骨细胞的增殖和分化作用,0 5 0g·L-1促使成骨细胞矿化结节的形成。

Methods The chemical hypoxia was induced by incubation of U251 cells with cobalt chloride.

以氯化钴模拟缺氧,采用RT-PCR、免疫细胞化学方法分别检测缺氧诱导因子-1α和血管内皮生长因子mRNA和蛋白的表达,鸡胚绒毛尿囊膜模型检测缺氧时胶质瘤细胞血管生成情况。

These were raised underinducing condition to test if any morphology and structure changes. Twenty two Arabidopsismutants had various changes in morphology and anatomical structures. Twenty mutants hadlow germination rate from 10% to 60%, of which 2 had survival rates at 0% and 50%. Fivemutants occurred structure changes in the hypocotyls or stem. One of these lines, namedarris-stem, showed some unique changes: slower growth rate in comparison with the wildtype from germination to florescence; serrated margin of leaf blades, spiral rosette; morebranch in the bottom part of the stem, shorter nodes, twisted stem and branch. There were oneor several arrises along the stem. Across sections of the arrises showed one or several compactcells lumps which were round, made up of several layers of cells, looked like vascular-bundle.

在诱导条件下,这些突变体表现出不同的形态和结构的变化,共有22个表型和结构发生变化。20个突变系发芽率或存活率较低,发芽率由10%—60%,其中2个突变系存活率分别为0%和50%。5个突变系在茎或下胚轴的结构上发生变化,其中一个突变系arris-stem发生如下表型变化:从真叶出现至开花结实的整个生长期,生长速度要明显比野生型拟南芥缓慢;叶缘有明显锯齿,莲座叶呈螺旋状排列;茎的基部有较多侧枝,侧枝间距明显缩短,茎有明显扭曲;突变体茎侧面有1至数条棱形突起,内部存在一至数个排列紧密的细胞团,由多层细胞呈环形排列,细胞壁明显加厚,内部存在管状分子,推测为维管组织。

These orient themselves so that a fluid-filled cavity, the blastocoel, forms inside the mass of cells

这些细胞进行自我调整,以致在细胞群内部形成一个充满液体的腔,即囊胚腔。

Methods Mouse endometrial epithelial cells were isolated and cultured,then it was cocultured with mouse embryos fertilized in vitro.

对小鼠子宫内膜上皮细胞进行分离、培养鉴定,然后与体外受精的小鼠胚胎进行共培养,观察共培养对小鼠胚胎卵裂率、孵化率以及囊胚期胚胎总细胞数的影响。

Cytokinin KT and 6-BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6-BA was more effective than KT.

研究了细胞分裂素在玉米愈伤组织诱导和植株再生中的作用,结果表明低浓度(0.2mg/L)的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生,6-BA的效果比KT更好。

Cytokinin KT and 6-BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6-BA was more effective than KT.

黄璐研究了细胞分裂素在玉米愈伤组织诱导和植株再生中的作用,结果表明低浓度(0.2mg/L)的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生,6-BA的效果比KT更好。

Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally.

从鸡胚中分离出背根神经节并制成神经细胞,将重组人睫状神经营养因子加入到细胞中继续培养64h后,用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性,从而定量测定重组人睫状神经营养因子的生物活性。

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