胚细胞

With the assistance of molecular probes for reactive oxygen species, the production and localization of ROS derived from embryo, starch and aleurone layer of maize seeds with different ages were investigated under a laser scanning confocal microscope and a luminescence spectrometer.

借助活性氧发光分子探针，利用荧光光谱和激光共聚焦扫描显微技术，在不同年份的玉米种子吸胀早期，对其胚、淀粉和糊粉层细胞活性氧生成进行了定量和亚细胞定位分析。

After 16 weeks growth, continuous and intact ameloblast, enamel, dentin, odontoblast was found.

生长16周的人牙胚，已经形成连续完整的成釉质细胞，牙釉质、牙本质和成牙本质细胞层。

Results It was demonstrated that β-TrCP expressed in oral epithelium, tooth bud, mesenchymal cell cytoplasm of ameloblast and edontoblast of different stage of tooth development.

结果β-TrCP在胚胎10.5、13.5、14.5、16.5、18.5d的小鼠牙胚上皮层与间充质层，以及出生后0、3、6d的小鼠成釉细胞与成牙本质细胞胞浆呈特征性表达。

So acertain concentration of auxins is important to the induction of embryonic callus, and cytokinins are cooperative in the process of somatic embryo genesis and embryoid growth.

因此，一定浓度的生长素是诱导紫叶酢浆草胚性愈伤组织的关键因素，细胞分裂素在体细胞胚的发生和发育过程中起增效作用。

So a certain concentration of auxins was important to the induction of embryonic callus. Cytokinins were cooperative in the process of somatic embryo genesis and embryoid growth.

因此，一定浓度的生长素是诱导紫叶酢浆草胚性愈伤组织的关键因素，细胞分裂素在体细胞胚的发生和发育过程中起增效作用。

Somatic embryogenesis usually suffers an dedifferentiation process in which some somatic cells are transitted to embryogenic cells from vegetative cells.

体细胞胚发生一般经历一个脱分化过程，即由营养生长的体细胞转变成生殖生长的胚性细胞。

In this study, in utero transfection plasmid DNA method was set up to embryonic mouse. The GFP expression vector pCAGGS-IRES-EGFP was microinjected into the lateral cerebral ventricle of E 14.5d mouse embryos through the uterine wall. Then, the 30 V square-wave pulse was delivered across the head for 5 times and interval 50m sec by electrodes in parallel with the median raphes.

本研究旨在通过子宫内电穿孔法，建立鼠胚大脑皮层体内基因转移体系：将带有绿色荧光蛋白的表达载体pCAGGS-IRES-EGFP显微注入胚胎期14.5 d的鼠胚侧脑室中，电极沿与脑中缝平行的方向夹持鼠脑，在电压30 V，脉冲时间50 ms，脉冲数5的条件下方波电击，将外源基因转移到室管膜层细胞。

RESULTS: FISH demonstrated that the male pronuclei in some one-cell embryos and the each nucleus in some two-cell embryos presented positive signals.

结果： 在单细胞胚和二细胞胚间期核内发现HBV DNA阳性杂交信号，RT-PCR可以扩增出HBx基因的特异条带，免疫荧光也可见到HBsAg阳性信号。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了&胚性芽&，确立了植物胚挽救的另一种形式。

METHODS: The osteoblasts were harvested from the frontal bone of 15-day SPF chicken embryos by the enzyme digestion, and treated with various mass concentrations of estrogen (0, 5, 10, 20, 100, 200, 400, 800, 2 000, 20 000 ng/L) and letrozole (0, 5, 10, 25, 50, 100, 250, 500, 1 000, 5 000 μg /L).

取15日龄SPF鸡胚，酶消化法获取鸡胚额骨成骨细胞，分别采用不同质量浓度雌激素(0，5，10，20，100，200，400，800，2 000，20 000 ng/L)和来曲唑(0，5，10，25，50，100，250，500，1 000，5 000 μg /L)处理。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。