胚细胞

Their ability to self-renew and differentiate into tissues of mesodermal origin (osteocytes, adipocytes, chondrocytes) and their lack of expression of haemopoietic molecules are currently the main criteria for isolation.

当前分离的主要标准是根据他们自我更新并向中胚层来源组织（骨细胞，脂肪细胞，软骨细胞）分化的能力以及缺乏造血相关分子表达的特点。

Human basic fibroblast growth factor is a single-chain heparin-binding polypeptide. It is a general regulatory mediator for different cells such as endothelial cells and for other cells of mesodermal and neuroectodermal origin, it stimulates cells proliferation, inducing or delaying differentiation.

人碱性成纤维细胞生长因子是一种单链的具有与肝素结合能力的多肽分子，它能够调节内皮细胞及其它来源于中胚层和神经外胚层等多种细胞的生长、分化、迁移和凋亡，同时是一种重要的血管生成因子，因此具有广泛的临床应用价值。

IntroductionBasic fibroblast growth factor is a kind of cell growth factor which has a great effect on proliferation and differentiation to mesodermic cells.

前言 碱性成纤维细胞生长因子是一种对中胚层来源的细胞有强烈促增殖和分化作用的细胞生长因子。

The adhesive capacity of differentiated cells that stably expressed E-cadherin was markedly enhanced. Compact cell connection and multi-layer growth state remained at 19 days. While non-transfected ESCs gradually changed from embryoid bodies into noncohesive cell populations.

稳定表达上皮型钙粘蛋白的分化细胞间黏附能力明显增强，并在19 d时仍能保持致密的细胞连接和多层生长状态，普通胚胎干细胞则由拟胚体结构逐渐分化为松散的细胞群落。

It is shown from 〓H-TdR incorporation and MTT that, rhBMP-2 could enhance the proliferation of cultured MC3T3 cells and new born rabbit periosteal osteoblast to some extent, but has little effect on the growth of NIH3T3 cells.

TdR参入法和MTT法测定结果表明，rhBMP-2对培养的鼠胚颅骨样细胞和新生兔骨膜细胞有一定的促增殖作用，而对NIH3T3细胞无明显作用。

In explants of W5, DNA and RNA content increased in both ovules and parenchyma. DNAcontent in proligerous cells decreased after 6 days of culture. Amylose increased inparenchyma gradually and big starch grains appeared in proligerous cells.

在 W5 上的外植体内胚珠和薄壁组织细胞的 DNA、RNA 都增加，第 6-9 天，增生的细胞 DNA 含量减少；薄壁组织中多糖含量一直增加，并且发现增生的细胞内布满了大的淀粉粒。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示，在卵母细胞和8细胞期胚胎，基因calm3和trm112l的表达量无显著性差异，而在囊胚期的表达量与前两个时期的表达量存在显著性差异，推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控，提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

The course mainly introduces basic principles and methods of experimental techniques of cell engineering, which includesmedia preparation, sterilization and aseptic technique, virus-free seedling production, plant rapid propagation, immature embryo culture, pollen and anther culture, cell culture, protoplast culture, somatic hybridization, plant transformation, and so on. The contents of this course follow the from easy

该课程主要介绍细胞工程特别是植物细胞工程的基本实验技术及其原理与方法，包括：培养基配制、灭菌及无菌操作技术、无病毒苗培育、植物快速繁殖、幼胚培养、花药及花粉培养、细胞培养、原生质体培养、体细胞杂交及植物遗传转化等。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

Objective: First,to study the effect of Ginseng water-solubility total protein on African green monkey kidney cells, murine normal fibroblast cells L_(929, human embryo lung diploid cells (2BS) and human throat cancer cell (Hep-2) in cell proliferation .

目的：研究人参水溶性总蛋白对非洲绿猴肾细胞Vero、小鼠成纤维细胞L_(929)、人胚肺二倍体细胞2BS、人喉癌细胞Hep-2增殖的影响。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷