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RESULTS: CaB was not detected during early odontogenesis from the dental lamina to the late c ap stage .Small amount of the protein first appeared in ameloblast and some of dif ferenting cells in the surface of dental papilla in early bell stage. As to late bell stage, the cytoplasm of ameloblasts and odontoblasts with secreted dentin was all stained strongly, as well as some of n uclei.

结果:人牙胚蕾、帽状期成釉器无CaB的表达;钟状早期成釉器的内釉细胞及部分牙乳头表面处于分化状态的细胞弱阳性染色;钟状晚期成釉器的成釉细胞、成牙本质细胞及部分胞核呈现强阳性染色。

In the process of nucleus degeneration, cell organella, for example, rough endoplasmic reticulum, amyloplast, and mitochondrion, maintained normally metabolic functions in cytoplasm, and nutrient substance was still synthesized and accumulated at the time of nuclear degeneration, the starchy endosperm cells carried out their functions until cell death simultaneously.

在核衰退的同时,其胞质中的粗面内质网、淀粉质体和线粒体等细胞器具有正常的代谢功能,细胞仍在合成并积累营养物质,淀粉胚乳细胞表现出一边衰退一边在行使其功能,直到细胞死亡。

GFP-labeled and non-labeled cells from X blastoderm were large and round with the same characters of morphology. The GFP protein was not expressed in CEF cell .Our results suggested that the CVH promoter regions have specific activity in stage X blastoderm.

实验结果显示:在胚盘细胞转染后12 h可观测到绿色荧光表达,转染后24 h荧光细胞增多,细胞形态均呈圆形、体积较大;在鸡胚成纤维细胞未检测到GFP表达。

①At the 8 weeks of fetalage, the ciliated, small mucous granule cells and rare basal cells were all differentiated from endoderm cubic cells, which was 3 weeks earlier than Gaillard's report.

①人胚胎8周时,内胚层细胞已分化出气管上皮的纤毛细胞、小粘液颗粒细胞、基底细胞很少,该结果比过去Gaillard等的报告提前3周。

Antipodal cells The three haploid cells found in the embryo sac of seed-bearing plants that migrate to the chalaxal end of the sac farthest from the micropyle.

反足细胞:胚囊三次分裂形成卵细胞,助细胞和极核等,其中位于合点端的三个单倍体细胞称反足细胞。

The result showed that mouse fetus liver cells condition medium,which can maintain survivance and proliferation of mouse EG cells,was better than the medium containing no cytokine,but worse than EG cell medium with 1 000 IU/mL of LIF.

同时,试验对比了从不同胎龄胎儿分离培养原始生殖细胞的情况,鉴定了EG细胞的生物学特性;EG细胞经体外培养,可以分化为神经样细胞、上皮样细胞和简单类胚体。

According to 5'flank regions and partial sequences of the MyoG gene in Sus scrofa, Bos taurus, Homo sapiens, and Mus musculus published in GenBank, the homogeneous primers were designed to amplify the promoter region of Gansu wapiti MyoG by Touch-Down PCR.

MyoD和MyoG是一类肌调节蛋白,其中MyoD代表肌源性的发生;MyoG诱导骨骼肌的最终分化,调控中胚层细胞分化为成肌细胞,再由成肌细胞融合为肌纤维这一过程,在肌细胞分化过程中起着中心调节作用,是唯一的不可替代的成肌调节因子[2]。

It also indicates that the expression of WT1 is regulated strictly in time and space, and the aberrant expression of WT1 may result in retarded differentiation of blastemal cell leading NR or nephroblastoma.

提示在散发性肾母细胞瘤发生中除WT1突变外,WT1表达延迟可能是其中起重要作用的机制之一;WT1基因与肾母细胞瘤的组织分化有关,可能诱导胚基细胞向上皮细胞方向分化。

The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%.The sperm-like cells achieved could activate bovine oocytes by sperm injection, and the development rate of blastosphere was 20.36%. Conclusions SSCs of newly -born mice can be induced and differentiated into sperm-like cells and are also capable of activating oocytes.

本实验通过体外诱导新生小鼠SSCs分化为精子样细胞,探讨不同诱导方法的诱导效率;采用显微注射法将精子样细胞注射到卵母细胞和去核卵母细胞,探讨其体外重构胚的发育能力,为SSCs体外分化研究打下基础。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体,昆明白小鼠MⅡ期卵母细胞为受体,研究了3%蔗糖处理卵母细胞去核后,供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

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