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All the results indicated:the molecular mechanism for embryoes abortion mediated by calcium may be relevant to some genes, which controlling assenergy transfering oxidoreduction system endomembrane system and cytoskeleton structure system ,transcripted or expressed unnomally.

由以上研究结果初步可以认为:受钙胁迫花生幼胚败育的分子机理可能是与早期幼胚内与能量转化、氧化还原系统运转、细胞内膜以及细胞骨架功能维持相关的功能基因的转录和表达异常有关。

But the transfer of 2—4 cell stage parthenogenetic embryos to the pseudopregnant recipients failed to induce preqnancy in Kunming mouse.

孤雌胚具有发育良好的内细胞团。但将2—4细胞期孤雌胚移植至假孕受体未获得妊娠。

Mast cells w ere isolated and purif ied from human embryo oral mucosa and were co-cultured with the fibrobl asts from human embryo oral mucosa.The rough endoplasmic reticula a nd mitchondria of OMFb were observed and analyzed.

采用分离纯化人胚口腔粘膜肥大细胞的介质溶液与体外培养的人胚口腔粘膜成纤维细胞共同培养,对OMFb进行形态计量分析。

Except on the testis tissues,the expression of ZFY gene were found on the kidney tissues of human embryo or rat, brain vesicles〔rat embryo 13 days〕,and sclerotome cells〔rat embryo 13 days〕 It is possible that because kidney and genital system come from same origin,ZFY gene expressed on the kidney tissues too, the expressive function of ZFY gene on the embryonic brain vesicles and sclerotome cells is not yet clear.

除了在睾丸组织上表达外,ZFY基因还在肾脏组织及鼠胚脑泡(胚胎13天),生骨节细胞(胚胎13天)上表达。在肾组织上表达可能是肾脏与生殖系统起源相同有关。在胚脑泡及生骨节细胞上表达其作用不清楚。

From its the depart in splanchnic organization arrives bacterium of 2 individual plant, pass bacteriological method appraisal, one individual plant is deputy pig bloodsucking bacili, another individual plant is a pig streptococcic; will send check sample book have an inoculation to marc-145 cell, can observe cellular pathological changes, the pcr of virus fluid classics of results detects, for positive of blue ear ill viru

从其内脏组织中分离到2株细菌,通过细菌学方法鉴定,一株为副猪嗜血杆菌,另一株为猪链球菌;将送检样本接种到marc-145细胞,能观察到细胞病变,收获的病毒液经pcr检测,为蓝耳病病毒阳性;将送检的样本接种spf鸡胚,收获的鸡胚尿囊液有血凝性,血凝试验和血凝抑制试验鉴定为猪副黏病毒。

To establish the method of isolating the osteoclastic cells from the chick embryo, osteoclasts were isolated from long bones of the 18-day old chick embryo and the cultured on a glass coverslip or a bone slice.

从18日龄鸡胚长骨中机械分离破骨细胞於盖玻片和骨片上培养,以建立鸡胚破骨细胞体外培养方法。

Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料,分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式,研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变,ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变,揭示了突变体对这些基因的巨大影响,反映了它们可能存在相互作用。

Megaspore mother cells undergo meiosis, which results in the formation of 4 megaspores in a row. The chalazal-most cell develops into an 8-nuclei embryo sac after 3 rounds of mitosis. A mature embryo sac is produced after cellularization and nuclear migration.

结果表明:1大孢子母细胞经过减数分裂形成直线排列的4个大孢子,其中珠孔端的3个大孢子退化,合点端的大孢子进行3次有丝分裂,形成八核胚囊, 8个核迅速细胞化,最终发育为成熟胚囊。

Methods The B6D2F1 hybrid mouse oocytes were parthenogenetic activated with strontium chloride(SrCI2) and cytochalasin B. The parthenogenetic blastocysts and morulas were seeded on MEF feeder respectively to examine the primary ICM appearance.and compare the derivation rate.

采用氯化锶联合细胞松弛素B激活B6D2F1杂交小鼠卵母细胞,所获得的囊胚与桑椹胚分别用于孤雌胚胎干细胞的建系,观察两者的建系成功率。

By chicken embryo, minimum concentration of medicine of directly inactivated test、 treatment test and preventive test is 7.5mg/ml、 62.5mg/mland 31.25mg/ml;By CEF, minimum concentration of resiting NDVF_(48)E_8 and moderate virulence strain is 10mg/ml、 50mg/ml and 25mg/ml. However,the result of complex prescriptionpreventing and curing NDVF48Ej$ is not ideal.

在鸡胚上抗病毒试验中,对三株病毒起直接灭活、治疗和预防作用的最低药物浓度分别为7.5mg/ml、62.5mg/ml和31.25mg/ml;在鸡胚成纤维细胞上抗病毒试验中,对强强毒株和中等毒力株起直接灭活、治疗和预防作用不引起细胞病变的最低药物浓度分别为10mg/ml、50mg/ml和25mg/ml。

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