胚细胞

Among the 20 samples of IGF- 1 group, a 8-cell stage embryo, a morula and two blastula were found as positive by the red colour appearance .

IGF上的表达 2～8细胞胚胎 18个，桑堪胚 1个，囊胚 2个，共计 ZI个胚胎用于＊F2 检测，阳性表达14个，其中强阳性表达7个，弱阳性表达7个，分布于各阶段胚胎，在胚胎细胞中呈现深、浅程度不同的橘红色染色。l级和 2级胚胎 IGFZmRNA的阳性表达差异有显著性。

After a continuous culture for 24 h, 77.1%(81/105)of the reconstituted embryos developed to 2-cell stage; The developmental rates of 4-celland morula were 45.7%(48/105) and 17.1%(18/105), respectively.

体外继续培养24h后，77.1％(81／105)的重构胚发育到2-细胞；4-细胞和桑椹胚的发育率分别达到45.7％(48／105)和17.1％(18／105)。

An attenuated vaccine strain of Muscovy Duck Reovirus, which was suitable for propagating in chicken embryo fibroblast, was obtained by serial passage alternately between Muscovy duck embryo fibroblast and CEF.

应用番鸭胚成纤维细胞和鸡胚成纤维细胞交替传代选育出适应CEF繁殖的番鸭呼肠孤病毒弱毒疫苗株。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测，最早在肢芽期胚胎中检测到2A3阳性信号；在膜内无节幼体期，2A3阳性血细胞平均直径约7μm，聚集成簇；在无节幼体Ⅰ期，血细胞形态更加完整清晰，平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现，这些细胞同样成簇存在，在其细胞质外围可见点状荧光。

At fertilization one male nucleus fuses with the gee nucleus to form the zygote, while the second male nucleus fuses with the primary endosperm nucleus to form a triploid cell, or more rarely a second diploid or a pentaploid cell that later gives rise to the ENDOSPERM.

受精作用发生时，一个精核与卵核融合形成受精卵，第二个精核与最初胚乳核融合，形成三倍体细胞，偶而也形成第二个二倍体或五倍体细胞，三倍体细胞最后发育为胚乳。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法］对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后，分别采用MTT试验、蛋白印迹技术和逆转录－聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

Chapter Ⅱ Xenopus Tbx6 Mediates Presomatic Mesoderm Cell Adhesion during Somitogenesis As a member of T-box gene family, Tbx6 is involved in somite cell fate decision and endows adhesion properties to mesoderm cells.

第二章 xTbx6在体节发生过程中介导了预定体节中胚层的粘连 xTbx6是T-box转录调控因子家族的成员，xTbx6参与体节细胞命运决定和赋予旁侧中胚层细胞特定的粘连特性。

The ovary of Phyllostachys praecox had one anatropous ovule. The crassinucellate ovule had diploid integuments. The monosporic embryo sac had one tetrad which turned on line and many antipodal cells. The synergid had the obvious filiform appratus.

3雷竹子房1室，内具1个倒生胚珠；双珠被，厚珠心；蓼型胚囊，单孢发育，线形大孢子四分体，具多个反足细胞，助细胞具明显丝状器。

Observation on the ultrastructure of the radicle cell of cabbage seeds indicated that the ultrastructure changed with the seed vigor reduced, such as the segregation of cytoplasm from the cell wall, the fragmentation of cell membrane, the vesiculation of protoplasm, the homogenization of chromation, the disintegration of cytoplasm, the distinguishing of nuclear membrane and nucleous ,and so on.

胚根细胞亚显微结构电镜观察结果，甘蓝种子通过人工老化活力降低后，胚根超微结构发生了变化，主要表现为：质壁分裂，细胞膜内陷或局部断裂，细胞质空泡化，细胞器难以辨认，染色质均质化，核仁和核膜的界限模糊等现象。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷