胚细胞

The effects of IGF-1 in different concentrations was determined on the development of blastocyst in hyperglycemic conditions (30.0 mol/L glucose) in vitro, as well as the apoptosis of embyro cells by using nuclear DNA double-dyed assays.

观察不同浓度胰岛素样生长因子-1(insulin-like growth factor-1， IGF-1)对体外高糖环境胚泡细胞发育的影响，利用细胞核DNA双染实验观察不同浓度IGF-1作用下胚泡细胞的凋亡。

The middle germinal layer of an early embryo,consisting of undifferentiated cells destined to become the mesoderm.

中胚层早期胚的中间胚芽层，由最终将发育成中胚层的未分化细胞组成

The middle germinal layer of an early embryo,consisting of undifferentiated cells destine d to become the mesoderm.

中胚层早期胚的中间胚芽层，由最终将发育成中胚层的未分化细胞组成

The microscopical observation showed that embryonal suspensor masses developed firstly into early stage proembryos,then into late stage proembryos.

显微观察表明，来自原生质体的胚性胚柄细胞团(ESM：embryogenic suspensor mass)，经早期原胚(ESP：early stage proembryos)阶段形成了后期原胚(LSP：late stage proembryos)。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The main research achievements on oogenesis and embryogenesis in mammals are described in this review,including the resumption of meiotic division;activation of MⅡ oocyte;development of parthenogenetic,gynogenetic,androgenetic and reconstituted embryos;and the genetic and reproductive potentials of chromosomes within the first and the second polar body.

本研究将论述哺乳动物卵子发生和胚胎发生的最新研究进展，包括：减数分列的启动， MⅡ期卵母细胞的激活，孤雌胚、雌核胚、雄核胚和重组胚的发育以及第一与第二极体染色体的遗传和生殖潜力。

Globular embryos developed cotyledonary embryos and regenerated plantlets when transferred to other mediums.

分化培养10d时形成多细胞原胚，20d时形成大量球形胚，30d时由具有一定分化的球形胚和少量心形胚组成。

It was confirmed that facultative apomixes is existed in coffee species based on emasculating treatment, cyto-genetic observe and SSR marker detection.

细胞遗传学观察发现咖啡四倍体类型材料(2n=4x=44)的多胚和多胚苗中含有天然的单倍体胚和单倍体小苗(2n=2X=22)；去雄处理后，可见子房膨大，其中5份材料获得了果实和种子，初步说明无融合生殖现象的存在；对小粒种品种间杂交F1代个体的SSR分析，发现多胚苗中存在与母本图谱相同且不携带父本特异带的后代以及携带双亲特异带的有性后代，表明子代有仅来自母本的无融合生殖个体分离出来。 3 建立了咖啡无融合生殖的分子鉴别方法。

The galactosyl oligosaccharides of raffinose series are widely distributed in seeds of many species and are localized in tissues that remain viable after desiccation, including the embryo and aleurone layer of cereals, cotyledons and axis tissues of legumes and other dicots.

棉子糖半乳糖苷系列寡糖广泛分布在许多种植物种子中，并存在于干燥后仍能保持活力的组织内，如禾谷类种子的胚及糊粉层，豆类及其他双子叶植物的子叶和胚轴组织等。棉子糖半乳糖苷系列寡糖在禾谷类种子的非自溶性中央胚乳中不合成，但存在于蓖麻种子的自溶性胚乳细胞中。

Mature and immature embryos of maize inbred line 87-1 were used as explants and calli were induced and cultured on improved N6 medium. The method of enzyme-linked immunosorbant assay was employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3) and cytokinins of immature, mature embryos and their calli during culture process.

以玉米自交系87-1的幼胚和成熟胚为材料，采用酶联免疫法测定了不同培养时间诱导的幼胚及成熟胚愈伤组织内源脱落酸、吲哚乙酸、赤霉素(GA3)和细胞分裂素含量的动态变化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失