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胚泡

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Histological structures of bursas of Fabricius were observed at the different developmental stages from chicken embryoes to chickens. The results were as follows. The bursa of Fabricius began to develop on the 13th day of post-hatching and its cell aggregated into the nodosity clique underneath the epithelium mucosae. On the 15th day of post-hatching, those cell cliques were surrounded by the flat epithelium and formed the basic structure of folliculus lymphaticus. The number of lymphocytes in the folliculus lymphaticus increased gradually, and the medulla and cortex of a majority of folliculus were not discriminated obviously. Until on the 7th day for chickens, the medulla and cortex of folliculus formed completely and the number of folliculus increased significantly. From 21 to 35 days old, the folliculus lymphaticus transformed from polygon into rectangle. Mucosa epithelial cells increased gradually and their cytoblasts arranged compactly into aline in the basilar part on the 35th day.

本研究观察了从鸡胚到出壳后的不同生长发育阶段法氏囊的组织结构,结果表明:胚胎13日龄时,法氏囊开始发育,粘膜上皮下形成结节状细胞集团;胚胎15日龄,聚集的细胞集团形成初始的淋巴滤泡;胚胎18日龄到出壳后1日龄,滤泡内淋巴细胞数量增多,多数滤泡髓质和皮质界限仍不明显;7日龄雏鸡法氏囊髓质皮质分界清晰,滤泡数量显著增多;21日龄到35日龄,滤泡由多边形逐渐转变成长方形;在35日龄时,粘膜上皮细胞逐渐增多,排列紧密,细胞核紧密排列成直线位于基底部。

Results: Mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. After ovum implantation ,the mast cells were comparatively less at the place of implantation. The number of mast cells of early and late pregnancy(1~5 dyas, 15~19 dyas) was apparently higher than that during middle pregnancy(P<0. 05). Stained by alcian blue-safranin, both red and reddish blue mast cells decreased gradually in number during pregnancy, but the proportion of blue mast cells was increased. Critical electrolyte concentration of mast cells decreased as time went on.

结果:(1)肥大细胞主要分布于子宫肌层,子宫内膜较少,且胚泡植人部位的肥大细胞明显少于非植入部位;(2)妊娠早期(孕1~5d)和妊娠后期(孕15~19d)肥大细胞数显著多于妊娠中期(P<0.05);(3)Alcian蓝藏红组化染色后,可见妊娠过程中红色和红蓝混合色肥大细胞逐渐减少;蓝色肥大细胞所占比例逐渐增大,孕9~19d肥大细胞几乎全部为蓝色;(4)肥大细胞临界电解质浓度值随妊娠时间的推移而变小。

Gonadorelin/therapeutic use ; polycystic ovarium syndrome/drug therapy ; fertilization in vitro ; embryo transfer

促性腺素释放激素/治疗应用;多囊卵巢综合征/药物疗法;受精;体外;胚泡移植

Howeer, at least one group has made it to the first step--creating a human blastocyst by inserting the nucleus of an adult skin cell into an enucleated oocyte Science, 28 April, p.

然而,至少有一个研究小组已经走出了第一步,通过将一枚**皮肤细胞的细胞核植入一枚去核的卵细胞培育了一个人类胚泡(科学,4月28日,516页)。

During peri-implantation, the decrease of the ECL and the PHA-L receptors may lead to the failure of implantation in the oviduct, and the increase of the UEA receptors at diestrus may contribute to the process of fertilization.

围植入期ECL和PHA-L受体表达下降,在输卵管内形成了不利于植入的微环境,可能是胚泡正常情况下不植入输卵管的原因之一;动情期UEA受体水平显著低于动情间期,有利于受精过程的正常进行。

During periimplantation, the decrease of the ECL and the PHAL receptors may lead to the failure of implantation in the oviduct, and the increase of the UEA receptors at diestrus may contribute to the process of fertilization.

围植入期ECL和PHAL受体表达下降,在输卵管内形成了不利于植入的微环境,可能是胚泡正常情况下不植入输卵管的原因之一;动情期UEA受体水平显著低于动情间期,有利于受精过程的正常进行。

Methods LN in mouse endometrium was shown and measured with indirect immunofluorescence techniques, immunohistochemical ABC method and image analysis system. The effect of LN on the implantation was studied by using LN-antibody intra-uterine injection.

应用间接免疫荧光法、免疫组织化学ABC技术及图像分析法进行LN定位和定量测定;利用子宫内注射LN抗体的方法探讨LN对小鼠胚泡植入的作用。

Inject PAK2-N teminal (PAK2-NT) mRNA to Xenopus oocyte,and observe germinal vesicle breakdown by fluorescence microscope.

荧光显微镜下比较PAK2-NT mRNA注射组和未注射对照组卵母细胞胚泡破裂发生。

PKA activators such as cAMP, 8 - Br -cAMP or phosphodiesterase inhibitor isomethyl butyl xanthine or purified PKA catalytic subunit all can inhibit germinal vesicle breakdown and meiotic maturation in mouse oocytes, also in Xenopus oocytes.

以小鼠卵母细胞为实验对象,给予PKA激动剂cAMP、8-Br-cAMP或磷酸二酯酶抑制剂IMBX均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。

It is known that ovulation and fertilization triggers embryonic development and endometrial differentiation by corpus luteum progesterone production.

在排卵周期中,子宫内膜仅在极短的时间内才允许胚泡植入,称为种植窗期,通常在月经周期的20-24d,即LH高峰后的第7-11d。

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