胚根

The effects of different concentrations of Cr(superscript 6+)(0, 10, 20, 50, 100 mg/L K2Cr2O7) on the content of Pro, malondialdehyde, membrane permeability, activities of protective enzyme SOD and POD in roots and epicotyls of maize seedlings were studied.

研究了不同浓度(0， 10， 20， 50， 100mg/L K2Cr2O7)Cr(上标 6+)胁迫对玉米幼苗根和上胚轴中脯氨酸和丙二醛含量、细胞膜透性、超氧化物歧化酶及过氧化物酶等保护酶活性的影响。

The results were as the follows: with the rising of Cr(superscript 6+) concentration, the Pro content in both root and epicotyls was first up and then down; the content of MDA and membrane permeability increased continuously; SOD activity deceased gradually and POD activity was first up and then down.

结果表明，随着Cr(上标 6+)胁迫浓度的增加，根和上胚轴中Pro含量呈先升后降的趋势，MDA含量和细胞膜透性则持续递增，SOD活性逐渐下降，POD活性先升后降。

Epicotyls were cutted longitudinally and cultured in the dark first,on the MS basal medium with 3 mg/L 6-BA and 30 g/L sugar,100% adventitious shoot induction and an average of 33.8 shoots per explant were obtained.The base of the adventitious shoots was treated with 1000 mg/ L IBA for 10 min,then placed onto the filter papers which ends dipped into liquid medium with...

上胚轴纵切结合前期暗培养，在MS附加6-BA 3mg/L、蔗糖30g/L培养基中，不定芽诱导率为100%，每个外植体平均诱导的不定芽数达33.8；不定芽在1 000mg/L IBA溶液中浸泡10min后，移入1/4MT+1/2MT+10g/L蔗糖的液体培养基中，采用滤纸桥法进行培养，生根率达100%，平均根数2.5条；移苗成活率达100%。

Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally.

从鸡胚中分离出背根神经节并制成神经细胞，将重组人睫状神经营养因子加入到细胞中继续培养64h后，用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性，从而定量测定重组人睫状神经营养因子的生物活性。

Fruticosa grown in clay sandy soil and clay soil was best, and they were significantly higher than that in other soils from 12th d in the test. The biomass of hypocotyls in 4 soils was similar, and that in forest soil was higher and its dry weight accumulated in hillside soil was more.

沙土和黏土中紫穗槐根的生物量最好，并在第12天的时候开始就明显高于其他土壤。4种土壤中的下胚轴生物量都差不多，以森林土的较高，而山坡土的积累的干重较多。

Receptor system of transformation have developed from meristems to callus receptor ; and the gene transformation methods have made some progress.

香蕉转化受体系统已从茎尖发展到胚状体和愈伤组织受体系统，遗传转化方法也在不断改进，从单一的方法发展为根癌农杆菌感染法与基因枪法的结合。

Northern blot analysis showed that the GFP gene was correctly expressed in the transgenic plants. Green fluorescence was observed in roots and immature embryos of transgenic plants by fluorescence microscopy.Progeny analysis showed that GFP insertions were inherited in T_2 generation with normal expression.Both Mendelian and non-Mendelian inheritances were observed in T_1 progenies of transgnic maize,and non-Mendelian inheritances were observed in T_2 progenies.

Southern blot结果表明GFP基因以低拷贝数、简单的方式(1～3条Southern杂交带)整合到玉米基因组；Northern blot结果表明GFP基因在转录水平上表达；在转基因植株的根和幼胚中观察到GFP表达；转基因植株的后代遗传分析表明GFP基因能够遗传至T_2代并正常表达，部分转基因株系中的GFP基因以孟德尔遗传方式传递给T_1代，但T_2代不符合孟德尔分离比。

The infection of rhizobia to rice was a dynamic process beginning with surface colonization of the rhizoplane (especially at lateral root emergence), followed by endophytic colonization within roots, and then ascending endophytic migration into the stem base, leaf sheath, and leaves where they developed high populations.

同时，根瘤菌还可以沿植物的表面从根到地上部分迁移。在植物的生殖生长阶段，内生根瘤菌仍然保持活动性，可以进入烟草子房的子房壁、胎座和胚珠内，暗示根瘤菌通过种子向子代垂直传播的可能性。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。