胚根

In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.

研究以凤仙花无菌苗胚芽段作为外植体，经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花，在60d左右实现了凤仙花离体培养再生植株并在试管内开花。

The cytogenetic toxic effects of different concentration of As2O3 within different time on the cell of Vicia Faba root tip were studied.

本文以蚕豆根尖为材料，研究不同浓度As2O3在不同的处理时间内对蚕豆胚根根尖的细胞遗传学毒性效应。

Different plant expression vectors had been respectively transferred into upland cotton cultivars Jinman12, Jinmian7, Xinluzao1 and Jihe321 via Agrobacterium tumfaciens transformation. These vectors carried cryIAc3 gene, which encodes Bacillus thuringiensis δ-endotoxin, under the control of chimeric OM promoter, CaMV 35S promoter, and cotton leaf curl virus RP promoter respectively; snowdrop lectin gene (Galanthus nivals agglutinin, gna) trans-regulated by rolC promoter controlled TGAla factor; and multivalent expression construct of both cryIAc3-cpti fusion gene and gna gene. A large number of transgenic plants and their progenies had been obtained.

为了提高外源基因的表达量，延缓害虫产生耐受性，本文通过将携带有分别在高效复合OM启动子，CaMV35S启动子及棉花曲叶病毒RP启动子控制下的苏云金杆菌杀虫毒蛋白基因cryIAc3；反式调节因子增强韧皮部表达的雪花莲外源凝集素(Galanthus nivals agglutinin，GNA)基因；以及同时含有cryIAc3、豇豆胰蛋白酶抑制剂基因和gna三价抗虫基因的植物表达载体，以根农杆菌介导法分别将这些表达载体导入了陆地棉新陆早1号、晋棉7号、晋棉12号和冀合321等我国西北棉花主要栽培品种，经体细胞胚再生获得了大批转基因再生植株。

Agrobacterium tumefaciens-mediated transformation system of Medicago truncatula cotyledonary-node explants Abstract The three-day-old seedlings of Medicago truncatula, which geminated in medium containing up to 5mg/L 6-BA, were used to prepare the cotyledonary-node explants which contained one cotyledon and 1-2mm of split hypocotyl. High-efficiency regeneration system of Medicago truncarula was developed by axillary shoot organogenesis of cotyledonary-node explants.

论文第二部分 Medicago truncatula遗传转化和植株再生体系的研究第一章根癌农杆菌介导的Medicago truncatula子叶节外植体转化系统的研究本文利用在高达5mg／L 6-BA的萌发培养基上生长3天的Medicago truncatula幼苗，制备获得由一片子叶和1-2mm切分的胚轴组成的子叶节外植体。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

RT-PCR analysis showed that GhMADS1 gene expressed in petals, stamens, ovules and fibers, but not in roots, stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral organs are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

RT-PCR analysis showed the GhMASD1 gene expressed in petals, stamens, ovules and fibers, but not in roots,stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral orgens are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

Roots are positively hydrotropic and hypocotyls negatively hydrotropic.

根主动向水而胚轴具有负向水性。

Somewhat the typical xeromophic structure of leaf became light mesophytic in nature.

污水排放不会改变土壤质地，对土壤pH、全盐量和有机质含量影响不显著；但导致根中形成层层数和木质部束数减少，而根系活力未受抑制，胚轴和茎的结构未发生变化，在一定程度上却使叶片典型的早生性质变得不强烈。

The prtein autophosphorylation assay showed that ZmCIPK31 possessed protein kinase properties and millimolar range of Mn2+ was effective cofactor.3. Expression analysis of ZmCIPK31 gene in maizeCertain ZmCIPK31 expression level in stem, root, embryo, filament, seed and leaf of maize were detected under normal growth conditions. And the relative expression level is the highest in filament.

蛋白自磷酸化试验表明ZmCIPK31具有激酶活性，且微量的Mn2+是有效的辅助因子。3、ZmCIPK31基因在玉米中的表达分析ZmCIPK31在正常生长条件的玉米的茎、根、幼胚、花丝、种子及叶中均有一定量的基础表达，且在花丝中的相对表达量最大。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。