胚根

Efficient usage of the main storage material in megagametophyte before it falls off from seedling is important for seed germination of Pinus edulis Engelm.. We examined and compared lipid usage in both megagametophyte and cotyledon with electromicroscopic technique, the length, dry weight, starch content and FBPase activities of each part of the seedling were measured and compared.

采用电镜技术观察了黑暗中萌发中食松子叶和提供营养的雌配子体中脂肪体和与脂肪利用有关的乙醛酸循环体的变化，测量比较了子叶、下胚轴、根的长度、干重、淀粉含量，以及果糖1，6二磷酸酶的活性变化，对贮藏物质脂类、淀粉的利用及与生长的关系进行了分析。

Up to now,there is no variety having the resistance to root-knot nematode obtained via ovule resuce in china.

目前，在我国还没有通过胚挽救培养技术获得的抗根结线虫的番茄品种。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The invention relates to a rapid breeding method which uses MS as the basic culture medium and respectively performs the tissue culture of a xuancheng pawpaw through inducing a sprout and a root.

一种用MS作基本培养基，分别通过芽和根诱导进行宣木瓜组织培养快速繁殖的方法，其特征在于采用宣木瓜种子子叶或种子胚进行组织培养。

Pekinensis . IAA at concentration of 0.4-1. 0 mg/L in solid MS medium incited many adventitious roots on hypocotyl segments. The earliest anatomic changes were observed on cut surface of hypocotyl segments under optical microscope 24 hours after IAA treatment: cytoplasmic and nuclear density became higher in a few of parenchytmatous cells adjacent to phloem in tissue of pericycle, followed by cell divisions.

在生长素诱导24h后，可借助显微切片观察到下胚轴切面附近明显的解剖学变化：首先是中柱鞘内靠近韧皮部的薄壁细胞的细胞质与细胞核变浓，染色加深，部分细胞分裂；随后是分裂的细胞团增大，逐渐形成根原基并分化出根冠。

Up to now, auxin has been the only phytohormone that polar transported.

在胚芽鞘和茎尖生长素是向下运输，在根部是向根尖运输。

Are preparatively cultured in the differentiation culture mediums(IBA 1 mg/L, BA0.4 mg/L) for two days, and AS is added in preparative culture medium and MSo bacterial liquid that has been centrifugated. The extents of colouration are not identical.

影响瞬时表达的因素很多，本实验主要对四个因素进行测试：预培养天数(1天、2天)、不同激素配比的分化培养(IBA 1.0mg/L BA 0.4mg/L、IBA 0.2mg/L BA 2mg/L)、是否加入乙酰丁香酮、外植体类型(子叶、真叶、下胚轴、根)。

Methods: NGF was prepared from mouse submaxillary glands by the way of elution with CM 52 column. The molecular weight and purification of NGF were detected by SDS-PAGE polyacrylamide gel electrophoresis.

以小鼠领下腺为原料，採用CM52多次洗脱的力法分离神经生长因子，SDS-PAGE电泳检测NGF的分子品质和纯度，採用鸡胚背根神经节培养方法检测NGF的生物活性。

Subsequently, gold nanoparticles were adsorbed onto the composite surface through the amino groups of thionine and chitosan for immobilizing CEA antibodies. Finally, horseradish peroxidase was employed to block the possible remaining active sites of nano-Au layer to avoid the non-specific adsorption.

利用壳聚糖和硫堇分子中大量的氨基固定纳米金并吸附癌胚抗体；最后，用辣根过氧化物酶封闭活性位点从而制得高灵敏电流型免疫传感器。

Callus originated from plumular axis, cotyledon and root of Arnebia euchroma.

新疆紫草的胚轴、子叶、根都产生愈伤组织。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。