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Various celeriac explants were in vitro cultured on MSO media added different ingredients in order to induce high quality somatic embryos suitable for making artificial seeds.

对根芹的不同外植体在附加不同成分的MS培养基上进行离体培养,以诱导适合于制作人工种子用的高质量体细胞胚。

Semi-quantitative RT-PCR analysis showed that ZF1 expressed in root, stem, leaf, flower, immature pod, and embryo of chickpea with different expression patterns.

半定量RT-PCR分析表明,ZF1在鹰嘴豆的根、茎、叶、花、幼荚和幼胚中均有表达,在茎和叶中表达较弱,为组成型转录因子。

There is no transition between root and epicotyl of dicotyledonous seedlings with epigeal germination, whereas only having a directly simple connection between the tissues with the same radially developmental direction.

真叶叶迹的木质部与下胚轴中靠近韧皮部的后生木质部或次生木质部连接。根与上胚轴之间不存在维管组织的过渡、转变,而只是在同样发育方向的组织中有一种直接的简单的连接

The results indicated that the treatment of irradiation restrained the growth and differentiation of protocorm, especially to roots.The LD50 is 67.23Gy, because the protocorm is a integrate embryoids and higher tolerance was found. Ploidy analysis showed that most of aberrant seedlings go with the change of DNA and they were not euploid. When the plants trearted by irradiation, some of aberrant seedlings appeared stem forfication, and leaves appeared chlorophyll-deficient or green-white.,probably due to some genes' expression or closing.

结果表明:辐照处理对种胚原球茎的生长和分化有抑制作用,尤其是对根的抑制更为明显;由于种胚原球茎的完整性好,耐受性强,其辐照半致死剂量(LD50)为67.23Gy;流式细胞分析表明,大多数外部形态发生改变的植株其细胞内DNA的倍性发生了改变,出现相当多的非整倍体;此外,辐照产生的变异苗出现茎分叉、叶片缺绿或白绿相间等现象,可能是由于某些基因的表达与关闭引起的。

Three donor wheat all show good character and had little genotypical difference in the aspect of inducement、differentiation、regeneration etc. They could all nice genotypical cultivars for wheat transformation. 3160 embryos have been used to transformation (960 embryos belongs to Atlas66, 1000 embryos belongs to EM12, 1200 embryos belongs to Scout66 respectively), At last, we got twenty-six of which were positive cultivars through PCR. The preliminary whole transformation frequency was 0.82 percent. Also the citrate synthase activities of the leaves of T_0 progeny show 5~9 higher than the control lines.

转化过程中分别使用了960个Atlas66、1000个EM12和1200个Scout 66幼胚(总记3160个),经抗性筛选、CS与标记基因的PCR检测初步表明有26株为阳性植株,总转化率为0.82%;同时进一步对T_0代植株叶片柠檬酸合酶以及T_1代植株经铝胁迫后对其根尖分泌物进行检测,结果在上述转化植株中检测到了柠檬酸合酶的活性,且转化材料比对照材料平均高出了5~9倍;同时铝胁迫后,T_1植株根尖检测到了苹果酸及柠檬酸的分泌。

Cotyledon and hypocotyl ' s rate and quamity are the most among these explams , and callus can be obtained in 10 days by cotyledon and hypocotyl . reversely it is difficult to indue callus with root , and the callus from root is lnde and easy to become browning . the calius obtained from leaf grows very slow and does not become browning uniill in 2 or 3 months

银杏的不同器官和组织都能够诱导出愈伤组织来,其中,子叶和胚轴10d左右全部愈伤化,诱导速度和诱导率均最高,根则很难诱导,愈伤组织很少,褐化很快;叶片诱导的愈伤组织,生长慢,褐化也慢,在培养基上保持两三个月而不褐化;胚乳的诱导时间也较长,需要30d左右。

Three major embryonic tissue systems,shoot apical meristem,root apical meristem,and the differentiation of procambial strands,were visible in torpedo-stage somatic embryo.

三种主要的胚性组织系统包括芽顶端分生组织,根顶端分生组织和原形成层束在鱼雷形胚阶段清晰可见。

In vegetative organs, BnPAP17-2 and BnPAP17-3 are expressed in root, hypocotyls and stem, while BnPAP17-1 expression is limited to root.

在营养器官中,BnPAP17-2和BnPAP17-3在根、下胚轴和茎中有一定程度的表达,而BnPAP17-1只在根中表达。

Based on the present histological and previous molecular biological studies, the special structure at the apical end is obviously different from the cervical loop giving rise to Hertwig's epithelial root sheath, in human, mouse and rat molar tooth germs.

作者所作的组织学和分子生物学实验结果表明,人类、大鼠及小鼠磨牙牙胚中这种根尖末端特殊的结构与颈环区Hertwig上皮根鞘结构明显不同。

The leaf esterase,endosperm esterase, root esterase and coleoptile esterase zymogram phenotypes in Set II alien addition Lines of wheat-wheatgrass, octoploids of wheat-wheatgrass, common wheat and wheatgrass were analyzed.

本文分析了小冰麦异附加系列II、八倍体小冰麦、普通小麦和天兰冰草的叶片酯酶、胚乳酯酶、根酯酶和胚芽鞘酯酶和酶谱表型。

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