胚后期的

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

结果表明：黄口荔枝螺卵囊高度平均为7.33 mm，卵粒数量平均为165粒；黄口荔枝螺的早期发育属间接发生型，受精卵呈椭圆形，卵裂为盘状卵裂；在卵囊内，胚胎发育包括卵裂期、囊胚期、原肠胚、膜内担轮幼虫、膜内面盘幼虫；幼虫发育包括面盘幼虫、后期面盘幼虫和匍匐幼虫。

Both the new peniculus 1 and the old haplokinety separated at the telophase.The two germinal rows germinal rowsfor both the daughter cells appeared almost at the same time;(2) The macronucleus became shorter and thicker, finally oblate during the division of the cell. Then the macronucleus restored its original shape, band-like form. The micronucleus were divided into two before the macronucleus division;(3) The original scopula and stalk contributed to the old cell. The new cell formed scopula gradually after the cell division.

同时，新仔虫的第一咽膜(P′1)也开始由老单毛基索复制，并在细胞分裂后期与老结构分离；(2)大核在虫体分裂过程中由长带状逐渐缩短变粗至扁圆形，于虫体即将分开时迅速拉长，然后分裂为二个新大核；小核分裂先于大核，在两仔虫口纤毛器即将分开时完成；(3)原帚胚及柄归属老仔虫，新仔虫的帚胚在虫体分裂后逐渐形成，柄内肌丝则在柄鞘形成后逐渐长出。

The microscopical observation showed that embryonal suspensor masses developed firstly into early stage proembryos,then into late stage proembryos.

显微观察表明，来自原生质体的胚性胚柄细胞团(ESM：embryogenic suspensor mass)，经早期原胚(ESP：early stage proembryos)阶段形成了后期原胚(LSP：late stage proembryos)。

The results were as follows: 1.Inoculated on the induction medium at the late uninucleate stage,the microspores could initiate embryogenesis by first divisions in both symmetric and asymmetric patterns,but the former was predominant.

通过观察玉米小孢子胚胎发生的细胞形态学特征，发现：离体培养条件下单核后期的花粉可以通过均等分裂和不均等分裂两条途径形成胚状体，与前人的研究结果一致。

The expression profiles analysis of 25 genes belonging to 8 types in different development periods of Y35 somatic embry showed, genes involved in transcription, signal transduction, cell fate, substance transport, cell and development regulation were active at early and middle stages of somatic embryogenesis, while at the stage of cotyledonary embryo maturation, the high level-expressed genes were mainly those related to storage protein, maturation and defense.

通过半定量和Real-Time PCR技术，对WRKY和MAPKK等8类25个差异表达基因在Y35体细胞胚不同发育时期的表达分析显示，转录、信号转导、细胞生长分裂、细胞命运、物质转运和多数发育调节相关基因在体细胞胚胎发生的早期或中期比较活跃，而进入子叶胚成熟后期则主要是储藏蛋白、成熟和防御相关基因大量表达。

During embryogenesis, CaM mRNA was expressedstronger in the endosperm cells than in the proembryo cells at earlier stage but it wasreversed at embryo differentiation stage.

胚胎发育早期，钙调素mRNA在胚乳细胞中的表达比原胚中强，而后期则在分化胚中比胚乳细胞中强。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Dorsal enclosure go along the blastokinesis anaphase andaccomplishes at the same time at 48th hour; The embryo eats up yolk unenveloped into themidgut before hatching.

背合在胚动的后期进行，在48h胚动与背合同时完成；在桔小实蝇卵孵化前，胚胎的发育主要是通过未包入中肠的卵黄供给营养。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

The results showed that there are two main fastigiums in death curve during the hatching penod, one is in 4-6 day and another is in 17-19 day. The rest embryo ages were divided into three phases by the two pinnacles and the difference of died embryo among three phases is not significant. The results in this paper is not incompletely consistent with commonly experience value.

结果表明，整个孵化期间，胚胎死亡主要有2个高峰，第1个高峰在孵化前期的4~6日龄，第2高峰期在孵化后期的17~19日龄，这2个胚胎死亡高峰期把剩下的各胚龄分为3个阶段：1~3日龄、7~16日龄、20~21日龄，这3个阶段的胚胎死亡皆差异不显著，本研究结果与一般经验值不完全一致。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。