胚叶

Results There were fetal type in 12 cases, embryonal type in 7 cases, anaplasia type in 3 and mixed type in 2. The cytoplasmic postive expression of CK, AFP, S-100 protein and Vimentin of epithelial tumor cells were found in 14, 10, 9 and 6 cases respectively. Positive staining of CEA, P53 and P16 of nuclei of epithelial tumor cells were found in 11, 9 and 7 cases respectively.Among the 12 cases who underwent surgical excision, 8 patients with fetal type survived over 5 years. Conclusions This study suggests that immunoreaction of hepatoblastomas are different in the histological subtypes.

结果 胎儿型12例，胚胎型7例，间变型3例和上皮间叶混合型2例；细胞角蛋白、甲胎蛋白、S-100蛋白和波形蛋白在肿瘤上皮细胞浆的表达分别为14例、10例、9例和6例，癌胚抗原、P53和P16蛋白在细胞核中的表达分别是11例、9例和7例；手术切除肿瘤12例中8例生存期超过5年者均为胎儿型。

Semi-quantitative RT-PCR analysis showed that ZF1 expressed in root, stem, leaf, flower, immature pod, and embryo of chickpea with different expression patterns.

半定量RT-PCR分析表明，ZF1在鹰嘴豆的根、茎、叶、花、幼荚和幼胚中均有表达，在茎和叶中表达较弱，为组成型转录因子。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Microspore Ye as the feather, small sporophyll sac formed by the polymerization, each polymer capsules formed by four small sporangia, small spores with dual airbags, and airbag Pinaceae pollen is similar to large sporophyll from the central axis and arranged in Brachypodium feather composition, Each Yibing has a shell bucket, each bucket containing shell upright ovules 10-12 months.

小孢子叶枝为羽状，小孢子叶由聚合囊组成，每个聚合囊由4个小孢子囊组成，小孢子具双气囊，与松科植物花粉的气囊相似，大孢子叶由中轴及羽状排列的短柄组成，每一柄上有一个壳斗，每个壳斗内有10—12个直立的胚珠。

Methods］ Taking euphylla,cotyledon and hypocotyledonary axis as explants,the influences on the callus induction rate and the growth dynamic of Dictamnus angustifoliu in different medium,hormone matching and carbon sources were investigated.

方法］以幼叶、真叶、下胚轴为外植体，研究不同培养基、激素配比、碳源对新疆白鲜出愈率的影响以及愈伤组织生长动态变化。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Radical leaves: stipules ovate, herbaceous, adnate to petiole; petiole sheathing at base; leaf blade imparipinnate; leaflets opposite, rarely alternate, broadly elliptic or obovate-oblong; cauline leaves few, with few leaflets, or simple and then margin 3-lobed or 2- or 3-serrate.

胚根叶：托叶卵形，草本，贴生于叶柄；叶柄具鞘的在基部；叶片奇数羽状；小叶对生，很少互生，宽椭圆形或倒卵形长圆形；很少的茎生叶，有很少传单，3浅裂的或单的然后空白边2或3使成锯齿波。

Fruiting pedicels erect or ascending, often subappressed to rachis, densely pubescent; racemes bracteate at least basally; stems prostrate or rarely ascending; uppermost leaves 1-pinnatisect or pinnatifid; fruit terete; ovules 10-16 per ovary

果梗直立或上升，通常近贴伏于轴，密被短柔毛；具苞片的总状花序至少基部；茎匍匐或很少上升；最上部的叶1羽状全裂的或羽状半裂；果圆柱状；每子房胚珠10-16 1 S。 annua 中亚羽裂叶荠

Young embryos can be observed from the prothalli 1 month after sowing.

原叶体受精后1个月内即可观察到从原叶体上生成的幼胚。

In addition, isozymes changes of SOD and POD were observed in groups treated with laser after seeds being soaked for 24 h and when embryonic buds emerged, while no change was observed in the group treated at the one-leaf stage.

SOD、POD同工酶的酶谱在浸种24小时和胚芽露头期经激光处理后改变，在一叶期处理后没有改变；CAT同工酶谱没有变化；在一叶期处理的三种同工酶谱都没有改变。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。