胚

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Phosphorylase mRNA was less in proembryos, but significantly increased in differentiation embryos accumulating transition starch.

原胚中的磷酸化酶mRNA表达较弱，到分化胚积累过渡性淀粉时才显著增加。

Phosphorylase mRNA wasless in proembryos, but significantly increased in differentiating embryos accumulatingtransition starch.

原胚中的磷酸化酶mRNA表达较弱，到分化胚积累过渡性淀粉时才显著增加。

College of Life Science, Wuhan University, Wuhan 430072On the basis of optimization of in situ RNA hybridization technique, Westudied the temporal and spatial expression of calmodulin and phosphorylasemRNA and its relation with fertilization and embryogenesis in rice as well as antherdevelopment in tabacco. We also constructed cDNA libraries of proembryos anddifferentiating young embryos in rice using microscopic isolation and RT-PCRtechniques.

本文在优化RNA原位杂交技术的基础上，研究了钙调素mRNA和磷酸化酶mRNA时空表达及其与水稻受精和胚胎发生以及烟草花药发育的关系；利用显微分离技术和RT-PCR技术建立了水稻原胚和分化幼胚cDNA文库。

Multiplication rate achieved with solid/liquid alternate manner. 7. The late stage proembryos were induced in EC of multiplication medium with 9,000 mg·L-1myo–inositol and 0.1 mg·L-1 IBA.

含有50 g·L~（-1）蔗糖和0.1 mg·L~（-1）IBA的DCR1培养基最适合白皮松体细胞胚的成熟诱导，该组合明显提高了白皮松体细胞胚的成熟频率。

If you do not know how to become free of pimples, wheat germs will effectively help you. The only thing you should do to eat twoorthree tables poons of what germs per day.

如果你不知道如何摆脱粉刺的困扰，小麦胚芽就是你的好帮手，你要做的只是每天吃上两三匙分量的小麦胚芽。

If you do not know how to become free of pimples, wheat germs willeffectively help you. The only thing you should do to eat two or threetables poons of what germs per day.

如果你不知道如何摆脱粉刺的困扰，小麦胚芽就是你的好帮手，你要做的只是每天吃上两三匙分量的小麦胚芽。

If you do not know how to become free of pimples, wheat germs will effectively help you. The only thing you should do to eat two or three tables poons of what germs per day.

A A1 i8 Y5 _0 o3 n z 如果你不知道如何摆脱粉刺的困扰，小麦胚芽就是你的好帮手，你要做的只是每天吃上两三匙分量的小麦胚芽。

The relationship between embryonic development and changes of endogenous hormones in litchi ovules was studied.

以兰竹荔枝胚胎发育时期的正常胚珠与败育胚珠为试验材料，研究了胚胎发育与内源激素的关系。

The leaf esterase,endosperm esterase, root esterase and coleoptile esterase zymogram phenotypes in Set II alien addition Lines of wheat-wheatgrass, octoploids of wheat-wheatgrass, common wheat and wheatgrass were analyzed.

本文分析了小冰麦异附加系列II、八倍体小冰麦、普通小麦和天兰冰草的叶片酯酶、胚乳酯酶、根酯酶和胚芽鞘酯酶和酶谱表型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。