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MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells.

方法组织块贴壁法培养人晶状体上皮细胞,通过倒置相差显微镜、透射电子显微镜,采用免疫细胞化学法分析人晶状体上皮细胞的生长特性。

In the paper, an efficient maize regeneration system was developed using mature embryos as explants.

本研究以7个玉米优良自交系为材料,研究了影响成熟再生的各种因素,建立了成熟高频植株再生体系。

Malt removed impurity in the hopper is feeded into spray machanism from the outlet of hopper, and hot water of 60-65℃ is sprayed evenly and at the same time, the wet material is feeded to suply machanisam in order to suply malt Plumule at suitable temperaure and quantity to the kibbling, mat shell can be maintained intergrity and plumule is kibbled into size. While kibbing , some water is added into te size and the size is pumed into the saccharifying pot

将料斗内除杂的麦芽,由料斗出口进入喷湿机构,以60-65摄氏度热水对其均匀地喷湿,并同时将湿料输送给供料机构,使供料机械能连续不断地为粉碎辊提供温度为宜,数量相当的麦芽,以保证粉碎辊正常工作,麦芽经粉碎后,麦皮保持破而不碎,乳磨成浆料,在粉碎的同时加一定的清水,随之搅拌调稀泵入糖化锅。

The result showed that the isolation of embryosac was carried out with fresh ovules,which were macerated in two designed mixture solution.

实验了7种多种酶组合的混合酶液,其中以配方3和配方4效果较好,3-4小时内,可以从新鲜的泡桐珠中将囊酶离出来。

During the first 3 days of sequence culture, SOF and granulosa cell enhanced the development of porcine parthenogenetic embryos and the cleavage rate (P.05) and the number of beyond 4-cell embryos. During the following 3 days. NCSU-23 supplemented with FCS and POEC supported the embryo to morulae/blastocysts.

在序贯培养的前3d,SOF培养基和颗粒细胞对胎的发育有促进作用,分裂率(P.05)和突破4细胞阻滞的数目显著增加,在培养的后3d,添加胎牛血清的NCSU-23和输卵管上皮细胞能支持较多胎发育到桑囊,桑囊的发育率为(59.5±3.2)%(P.05)。

Confocal microscope detection experiments showed that, in morulae and blastocysts, with theincreasing concentration of BPA, embryo of cytochrome C release rate increased. This showedthat the cytochrome C involved in BPA-induced the embryos apoptosis process. This processis regulated by the mitochondrial pathway.

激光共聚焦显微镜检测细胞色素C结果显示:在桑椹期和囊期,随着BPA处理浓度的升高,释放细胞色素C的胎比率逐渐增加,说明细胞色素C参与了BPA诱导胎细胞凋亡的过程,这一过程是通过线粒体通路来调节的。

The study was conducted at 25℃ to cryopreserve the Australia Boer goat blastocysts and compacted morulae by one or two-step straw (Experiment 1) and OPS methods (Experiment 2) with vitrification solutions (EFS30, ESF40, EDFS30 and EDFS40) or by slow freezing (Experiment 3) with cryopretectant (1.5 mol/L EG).

在25℃室温下,采用细管法和OPS法,以不同浓度的EFS、EDFS为玻璃化冷冻液,对澳洲波尔山羊致密桑椹和囊进行玻璃化冷冻保存。同时利用1.5mol/L EG为抗冻保护剂对胎进行常规法冷冻保存。

The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

结果表明波尔山羊卵母细胞和早期胎都是端粒酶阳性;依据电泳条带在凝胶成像系统下的光密度计算相对总产品产量定量比较发现,卵母细胞最低为25.348,4-细胞为273.832,至8-细胞又降低到56.117,随后快速升高,桑椹为251.111,囊端粒酶活性最高为519.46。

If you're making hamburger patties to freeze, cut sections of the liner and put them in between the patties so they don't stick together.

如果你要做些汉堡包的面冷冻起来,把包装袋剪开隔在中间,面就不会粘在一起。

Used of the ovules or placentas in flowering plants.

用作开花植物的球或

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。