胚

The genome of avian adeno-associated virus was cloned for construction of gene transfer vectors. AAAV was propagated in special pathogen-free chicken embryo using the chicken embryo lethal orphan virus as the helper virus. The double-stranded genomic DNA was extracted from precipitated AAAV viron and cloned into pCR 2.1 vector.

为了克隆禽腺联病毒(Avian adeno-associated virus， AAAV)全基因组用于构建基因转移载体研究，以鸡胚致死孤儿病毒作为辅助病毒与AAAV共接种SPF鸡胚进行AAAV的增殖，将AAAV约4.7 kb双链基因组DNA与pCR2.1载体连接，构建了含AAAV全基因组的重组质粒pAAAV并进行了测序。

Put the seeds into wettish sand under temperature of 25,20,15,10,5 ℃,observe the shape changing of embryo and measure its length every other 10 days.

在25，20，15，10，5℃下层积处理明党参种子，每隔10 d观察种子胚的形态变化，并测定种子的胚长和种子霉烂率。

Vacuum tank and the equipment of 120℃ high temperature and 400Pa high pressure keeps GSH in corn plumule, Vitamin E and C and some trace nutrients has the function of enhancing human metabolism, regulating the nervous system, making the skin smooth and delicate, slowing down the appearance of wickless.

帕伦克的切割技术保留了95%的玉米胚芽，真空罐装和120度高温400帕高压杀菌设备锁住了胚芽中的谷肽甘胱，维他命E和C等微量营养元素，有增强人体新陈代谢，调节神经系统功能，使皮肤细嫩光滑，抑制，延缓皱纹等作用。

We systematically studied somatic and zygotic embryogenesis and its cytological process. This is the first report on these subjects till now.

本文首次对水曲柳的体细胞胚胎诱导和合子胚与体细胞胚发育的细胞学过程作了系统研究。

The different development stage of immature zygotic embryos have significant effect on.

7月30日和8月10日采集的白皮松成熟合子胚很难诱导出胚性愈伤组织。

Pale yellow, brown and white translucent calli were produced from zygotic embryos which were stored at 4 to 6℃ for 1 to 3 months and then cultured on medium LP+2mg/L 2, 4-D+1mg/L 6-BA in the dark for one month. Only white trenslucent calli were embryogenic.

合子胚在4～6℃低温条件下保存1～3个月后，接种于LP+2mg/L 2，4-D+1mg/L 6-BA的培养基上，黑暗条件下培养1个月后，产生浅黄色、褐色和白色半透明三种愈伤组织，其中白色半透明愈伤组织是胚性愈伤组织。

More efficient induction of embryogenic cultures was achieved from the immature zygotic embryos sampled on June 30, Inducing rates reached 97.22%.

0.3 mg·L~（-1）2，4-D+0.2 mg·L~（-1）NAA为胚性愈伤组织保持和增殖的最佳激素组合浓度。2，4-D与NAA的交互作用对胚性愈伤组织增殖存在显著影响。

Develop and implement value stream processes (seed generation,inoculation,incubation,candling,chilling,harvesting,clarification,UV treatment,inactivation).

制定和实施生产流程（鸡蛋接收，接种，孵化，照胚，冷胚，收获，澄清，紫外处理，灭活），保证所有的生产活动都被列示和了解，在保证质量和安全的前提下实现生产效率的最优化

One virus isolate was identified from the diseased chicken with a respiatory symptoms on some chicken farms in Anhui province.The artificially infected chickens appeared a transient respiratory syndromes, and the kidney of the death chickens were pale, flower-streak,swelled and has a deposes of uric acid salts.

从安徽省巢湖地区鸡群中出现的以肾肿大为主要特征的呼吸道疾病的病例中分离到1株病毒，易感雏鸡，病死鸡出现肾肿大、苍白呈花斑状、大量尿酸盐沉积；鸡胚连续盲传至第5代时开始出现死亡和侏儒胚。

A new method for enucleating MⅡ nucleus in mouse and rat was induced; Living births were obtained from IVF of mouse oocytes reconstructed by transfer of metaphase Ⅱ chromosomes. For the interspecies nuclear transfer between mouse and rabbit, the reconstructed embryos could develop into blasocyst in vitro.

在不同品系小鼠卵母细胞核质交换移植中将电融合的重构卵进行IVF，经胚胎移植后顺利产仔；在异种核移植方面，小鼠核与兔卵母细胞的异种重构胚在体外培养条件下发育至囊胚阶段。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。